Compositional changes in human gut microbiota reveal a putative role of intestinal mycobiota in metabolic and biological decline during aging

Q3 Medicine

Nutrition and Healthy Aging Pub Date : 2021-10-26 DOI:10.3233/nha-210130

M. T. Al Bataineh, A. Alzaatreh, R. Hajjo, B. H. Banimfreg, N. Dash

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引用次数: 3

Abstract

BACKGROUND: Age-related alterations in the composition and function of gut microbiota may influence human health and disease mechanisms. However, connections between compositional changes in gut bacterial and fungal communities, and their role in the aging process, remain poorly understood. OBJECTIVE: Compare the gut microbiota and mycobiota composition in different age groups and evaluate the functionality. METHODS: In this study, we performed 16S rRNA and ITS2 gene-based microbial profiling analysis and shotgun metagenomics using the NextSeq platform. RESULTS: We observed a shift in compositional changes of human gut microbiota with age. Older individuals revealed a significantly different gut microbiota profile compared to younger individuals. For example, gut microbiota composition of the older individuals showed increase in genera Bacteroides, Blautia, Ruminococcaceae, and Escherichia coli. Additionally, older individuals had significant reduction in fungi belonging to saccharomyces cerevisiae and candida albicans in comparison to their younger counterparts. Moreover, metagenomics functional profiling analysis using shotgun metagenomics sequencing data showed substantial differences in the enrichment of 48 pathways between the young and older age groups. Metabolic pathways such as amino acid biosynthesis, carbohydrate metabolism, cell structure biosynthesis and vitamin biosynthesis were declined in the older age group, in comparison with the younger individuals. CONCLUSIONS: The identified differences provide a new insight to enrich our understanding of age-related changes in gut microbiota, their metabolic capabilities, and potential impact on health and disease conditions.

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人类肠道微生物群的组成变化揭示了肠道分枝杆菌群在衰老过程中代谢和生物衰退中的假定作用

背景：肠道微生物群组成和功能的年龄相关变化可能影响人类健康和疾病机制。然而，肠道细菌和真菌群落的组成变化及其在衰老过程中的作用之间的联系仍知之甚少。目的：比较不同年龄组的肠道微生物群和分枝杆菌群组成，并评估其功能。方法：在本研究中，我们使用NextSeq平台进行了基于16S rRNA和ITS2基因的微生物图谱分析和鸟枪宏基因组学。结果：我们观察到人类肠道微生物群的组成变化随着年龄的增长而发生变化。与年轻人相比，老年人的肠道微生物群特征明显不同。例如，年龄较大的个体的肠道微生物群组成在拟杆菌属、白露菌属、瘤胃球菌科和大肠杆菌属中有所增加。此外，与年轻人相比，老年人属于酿酒酵母和白色念珠菌的真菌显著减少。此外，使用霰弹枪宏基因组学测序数据进行的宏基因组学功能分析显示，年轻人和老年人在48种途径的富集方面存在显著差异。与年轻人相比，老年组的氨基酸生物合成、碳水化合物代谢、细胞结构生物合成和维生素生物合成等代谢途径有所下降。结论：确定的差异提供了一个新的视角，丰富了我们对肠道微生物群与年龄相关的变化、它们的代谢能力以及对健康和疾病状况的潜在影响的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nutrition and Healthy Aging Agricultural and Biological Sciences-Food Science

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： Nutrition and Healthy Aging is an international forum for research on nutrition as a means of promoting healthy aging. It is particularly concerned with the impact of nutritional interventions on the metabolic and molecular mechanisms which modulate aging and age-associated diseases, including both biological responses on the part of the organism itself and its micro biome. Results emanating from both model organisms and clinical trials will be considered. With regards to the latter, the journal will be rigorous in only accepting for publication well controlled, randomized human intervention trials that conform broadly with the current EFSA and US FDA guidelines for nutritional clinical studies. The journal will publish research articles, short communications, critical reviews and conference summaries, whilst open peer commentaries will be welcomed.