Antioxidant, Antibacterial, α-Amylase, and α-Glucosidase Inhibition, and Anti-inflammatory Activities of Mimosa rubicaulis Lam

Q2 Pharmacology, Toxicology and Pharmaceutics
A. Adhikari, D. Shrestha, Sushma Thapa, Tamlal Pokhrel, Bishnu Bahadur Sinjali, J. Baral
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Abstract

The use of medicinal plants as supplemental or alternative medicine is widespread around the world. For the development of new drugs, studies on these medicinal plants that include pharmacological and toxicological assessments are crucial. This work aimed to find the total phenolic and flavonoid content, antioxidant, antibacterial, and antidiabetic potential of the traditionally used medicinal plant Mimosa rubicaulis Lam. The in vitro antidiabetic potential of methanolic extract and its fractions of the roots of M. rubicaulis were performed via enzyme (α-glucosidase and α-amylase) inhibition assays. Antioxidant and anti-inflammatory activities were carried out using 2,2 Diphenyl-1-picrylhydrazyl (DPPH), and reactive oxygen species (ROS) inhibiting methods. Well diffusion method is applied for antibacterial activity. Autodock vina was used for molecular docking. The crude extract reported the highest inhibition activity against α-glucosidase with an IC50 value of 10.29 ± 0.35 μg/mL compared to the standard acarbose IC50 value of 5.653 ± 0.29 µg/mL. Similarly, the ethyl acetate (EA) fraction disclosed significant inhibition against α-amylase with an IC50 value of 108.7 ± 0.66 μg/mL compared to the standard drug’s IC50 value of 6.01 ± 0.14 µg/mL. Likewise, the EA fraction showed the maximum antioxidant activity with an IC50 value of 11.89 ± 1.05μg/mL among the crude extract and its fractions. Mimosa rubicaulis was found to have α-glucosidase and α-amylase inhibition, anti-inflammatory, and antibacterial activity. To the best of our knowledge, this is the first report of α-glucosidase and α-amylase inhibition activity of this plant. Further studies on this plant are required to isolate potent compounds.
含羞草抗氧化、抗菌、α-淀粉酶和α-葡萄糖苷酶抑制及抗炎活性研究
药用植物作为补充或替代药物在世界各地广泛使用。对于新药的开发,对这些药用植物进行包括药理学和毒理学评估在内的研究至关重要。本工作旨在了解传统药用植物含羞草的总酚类和类黄酮含量、抗氧化、抗菌和抗糖尿病潜力。通过酶(α-葡萄糖苷酶和α-淀粉酶)抑制试验,研究了茜草根甲醇提取物及其组分的体外抗糖尿病潜力。采用2,2-二苯基-1-苦基肼(DPPH)和活性氧(ROS)抑制方法进行抗氧化和抗炎活性。应用井扩散法进行抗菌活性测定。Autodock-vina用于分子对接。粗提取物对α-葡萄糖苷酶的抑制活性最高,IC50值为10.29±0.35μg/mL,而阿卡波糖的标准IC50值则为5.653±0.29μg/mL。类似地,乙酸乙酯(EA)组分显示出对α-淀粉酶的显著抑制作用,与标准药物的IC50值6.01±0.14µg/mL相比,IC50值为108.7±0.66μg/mL。同样,EA组分在粗提取物及其组分中显示出最大的抗氧化活性,IC50值为11.89±1.05μg/mL。含羞草具有抑制α-葡萄糖苷酶和α-淀粉酶、抗炎和抗菌活性。据我们所知,这是该植物对α-葡萄糖苷酶和α-淀粉酶抑制活性的首次报道。需要对这种植物进行进一步的研究以分离出有效的化合物。
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来源期刊
Current Bioactive Compounds
Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.90
自引率
0.00%
发文量
112
期刊介绍: The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.
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