Jian-Pi-Yi-Shen drug-containing serum promotes proliferation and activity of mouse osteoblasts

IF 0.6 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Bioscience Journal Pub Date : 2023-06-09 DOI:10.14393/bj-v39n0a2023-64818

Yu Cai, Xiaochen Liu, Liang Ma, Wen-Ting Lv, Weiguo Wang, Xingcai Zhang

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引用次数: 0

Abstract

Jian-Pi-Yi-Shen (JPYS) decoction is a traditional Chinese herbal formula. The present study aimed to investigate the effect of JPYS drug-containing serum on the proliferation and activity of mouse osteoblasts. Sprague‒Dawley rats were fed JPYS decoction, calcium supplement, or normal saline for 6 weeks, and the serum was collected. Mouse osteoblasts were treated with JPYS drug-containing serum, calcium supplement serum, or blank control serum. Cell proliferation was assayed using thiazolyl blue tetrazolium bromide. Levels of alkaline phosphatase, nitric oxide, and nitric oxide synthase in the culture medium were measured. The JPYS drug-containing serum significantly improved the proliferation of osteoblasts compared to the blank control serum and the calcium supplement serum. It also significantly increased the levels of alkaline phosphatase in the culture medium compared to the blank control serum and the calcium supplement serum. Treatment with JPYS drug-containing serum for 48 h and 72 h significantly increased the nitric oxide (NO) concentration in the culture medium compared to the blank control serum. The NOS activity of the osteoblasts was significantly increased by JPYS drug-containing serum compared to blank control serum and calcium supplement serum. All these results were enhanced that the JPYS decoction promotes the proliferation and activity of mouse osteoblasts. These effects may be the underlying mechanisms of JPYS decoction in treating osteoporosis.

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健脾益肾药血清对小鼠成骨细胞增殖和活性有促进作用

健脾益肾汤是一种传统的中药配方。本研究旨在探讨jjpys含药血清对小鼠成骨细胞增殖和活性的影响。Sprague-Dawley大鼠分别饲喂JPYS汤剂、补钙剂或生理盐水6周，采集血清。用jjpys含药血清、补钙血清或空白对照血清处理小鼠成骨细胞。用噻唑蓝四唑溴铵检测细胞增殖。测定培养基中碱性磷酸酶、一氧化氮和一氧化氮合酶的水平。与空白对照血清和补钙血清相比，JPYS含药血清显著促进成骨细胞的增殖。与空白对照血清和补钙血清相比，培养基中碱性磷酸酶水平也显著提高。与空白对照血清相比，jjpys含药血清处理48 h和72 h显著增加了培养基中一氧化氮(NO)的浓度。与空白对照血清和补钙血清相比，jjpys含药血清可显著提高成骨细胞NOS活性。这些结果进一步证实了jpyys汤对小鼠成骨细胞的增殖和活性有促进作用。这些作用可能是jpyys汤治疗骨质疏松症的潜在机制。

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来源期刊

Bioscience Journal Agricultural and Biological Sciences-General Agricultural and Biological Sciences

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

48 weeks

期刊介绍： The Bioscience Journal is an interdisciplinary electronic journal that publishes scientific articles in the areas of Agricultural Sciences, Biological Sciences and Health Sciences. Its mission is to disseminate new knowledge while contributing to the development of science in the country and in the world. The journal is published in a continuous flow, in English. The opinions and concepts expressed in the published articles are the sole responsibility of their authors.