The assessment of genetic diversity and population structure of endemic Scutellaria yildirimlii (Lamiaceae) for conservation purposes

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.55730/1300-008x.2759

Merve Yıldırım, G. Tug, A. Yaprak

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Abstract

: Scutellaria yildirimlii is an endangered perennial endemic species from Turkey and belongs to the family Lamiaceae, which has a high number of medicinal and aromatic plants. It is crucial to determine genetic diversity and population structure of this species for its conservation measures; therefore, 111 individuals from five natural populations of S. yildirimlii were investigated by using 15 inter simple sequence repeats (ISSR) primers. The percentage of polymorphic loci (PPL), Nei’s gene diversity (H), and Shannon’s information index (I) at the species and population-level were determined as 93.9%, 0.183, 0.292 and 56.5%, 0.158, 0.242, respectively. The analysis of molecular variance (AMOVA) showed that there is more genetic variation within populations (84%) than among populations (16%). Nei’s differentiation coefficient (G ST = 0.144) was determined to be moderate, which was confirmed by the level of genetic differentiation among populations. Gene flow (N m = 2.984) showed high genetic exchange among populations. Pairwise genetic distance values among populations ranged from 0.0219 to 0.0512. The Mantel test revealed a statistically significant positive correlation between genetic and geographical distance (r = 0.493, p < 0.001). The Unweighted Pair Group Method with Arithmetic Averages (UPGMA) dendrogram and Principal Coordinate Analysis (PCoA) showed similar results. STRUCTURE analysis (ΔK = 3) revealed that the K test yielded maximum peaks for 3 clusters. Based on the findings, measures for genetic conservation and management of this species were presented.

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黄芩科特有植物黄芩的遗传多样性和种群结构评价

：伊氏黄芩是土耳其濒危的多年生特有物种，属于Lamiaceae科，该科有大量药用和芳香植物。确定该物种的遗传多样性和种群结构对其保护措施至关重要；因此，利用15个ISSR引物对来自5个伊尔迪里姆氏乳杆菌自然种群的111个个体进行了研究。在物种和种群水平上，多态位点的百分比（PPL）、Nei’s基因多样性（H）和Shannon’s信息指数（I）分别为93.9%、0.183、0.292和56.5%、0.158、0.242。分子变异分析（AMOVA）表明，群体内部（84%）的遗传变异比群体之间（16%）更多。Nei的分化系数（GST=0.144）是中等的，这是由群体间的遗传分化水平所证实的。基因流（N m=2.984）显示出群体间的高度遗传交换。群体间的成对遗传距离值在0.0219到0.0512之间。Mantel检验显示遗传距离与地理距离呈正相关（r=0.493，p<0.001）。算术平均未加权配对组法（UPGMA）树状图和主坐标分析（PCoA）显示了类似的结果。结构分析（ΔK=3）表明，K测试产生了3个团簇的最大峰值。根据研究结果，提出了该物种的遗传保护和管理措施。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.