Application of reliable cost-effective strategy for analysis of mycotoxins in corn-based foods with HPLC-FLD after multi-toxin immunoaffinity clean-up

IF 1.1 4区化学 Q3 CHEMISTRY, MULTIDISCIPLINARY

Macedonian Journal of Chemistry and Chemical Engineering Pub Date : 2022-06-22 DOI:10.20450/mjcce.2022.2422

B. Stojanovska Dimzoska, Zehra Hajrulai Musliu, Risto Uzunov, A. Angeleska, K. Blagoevska, Radmila Crceva Nikolovska, G. Ilievska, Elizabeta Dimitrieska Stojkovic

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引用次数: 3

Abstract

A different, reliable, and cost-effective strategy for the analysis of aflatoxins, ochratoxin A, and zearalenone in corn-based foods was proposed, including one multi-toxin immunoaffinity column (IAC) sample preparation and three different high-performance liquid chromatography fluorescence detection methods. The analytical procedures were tested and verified, keeping in mind their occurrence at trace levels in corn-based foods. With the validation of the proposed multi-toxin IAC methodology and comparison of the performance characteristics with methods using a single-toxin IAC, we confirmed the reliability of the multi-toxin IAC procedure versus the single-toxin IAC. The methods were validated by revealing satisfactory performance characteristics; for example, the obtained values of limit of detection were significantly lower than the maximum limits for all mycotoxins of concern. In addition, the recovery values were between 70.9 % and 106.1 % for all mycotoxins of interest, with precision values lower than 10.5 %.

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多毒素免疫亲和清理后HPLC-FLD分析玉米基食品中真菌毒素的可靠经济策略的应用

提出了一种不同的、可靠的、成本效益高的玉米食品中黄曲霉毒素、赭曲霉毒素A和玉米赤霉烯酮分析策略，包括一种多毒素免疫亲和柱（IAC）样品制备和三种不同的高效液相色谱荧光检测方法。对分析程序进行了测试和验证，记住它们在玉米类食品中的微量含量。通过对所提出的多毒素IAC方法的验证，以及与使用单一毒素IAC的方法的性能特征的比较，我们证实了多毒素IACs程序与单一毒素IACs的可靠性。通过揭示令人满意的性能特征来验证这些方法；例如，所获得的检测极限值显著低于所有关注真菌毒素的最大极限。此外，所有感兴趣的真菌毒素的回收率在70.9%和106.1%之间，精密度值低于10.5%。

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来源期刊

Macedonian Journal of Chemistry and Chemical Engineering 化学-工程：化工

CiteScore

1.60

自引率

20.00%

发文量

审稿时长

>12 weeks

期刊介绍： Macedonian Journal of Chemistry and Chemical Engineering (Maced. J. Chem. Chem. Eng.) is an official publication of the Society of Chemists and Technologists of Macedonia. It is a not-for-profit open acess journal published twice a year. The journal publishes original scientific papers, short communications, reviews and educational papers from all fields of chemistry, chemical engineering, food technology, biotechnology and material sciences, metallurgy and related fields. The papers published in the Journal are summarized in Chemical Abstracts.