Effect of curcumin on mitochondrial gene and high mobility group box 1 protein in sepsis myocardial injury of rats

Q4 Nursing

中华急诊医学杂志 Pub Date : 2019-09-10 DOI:10.3760/CMA.J.ISSN.1671-0282.2019.09.008

Yanjun Liu, Ying Yu, Xinping Zhang, Shuangjie Li, Yimin Zhu, Zhenghui Xiao, Xiulan Lu, Haipeng Yan

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Abstract

Objective To observe the effect of curcumin on myocardial mitochondrial related gene (mtATP6), high mobility group box 1(HMGB1) mRNA expression and plasma HMGB1 level in rats with sepsis, and to explore a new strategy for protection of myocardial injury in sepsis. Methods A rat sepsis model was established by cecal ligation and puncture (CLP). Thirty male Sprague-Dawley rats were randomly (random number) divided into the sham operation group (sham group), sepsis group (CLP group), and curcumin intervention group (Cur group), with 10 rats in each group. Rats in the Cur group were treated with curcumin by intraperitoneal injection at 100 mg/kg, and normal saline was injected into rats in the sham and CLP groups. Five rats were randomly (random number) sacrificed at 6 h and 24 h after modeling. The pathological changes of myocardial tissue were observed under light microscope. The levels of CK and CK-MB in serum were detected. The HMGB1 level in plasma was detected by ELISA. Real-time PCR was used to detect the expression of HMGB1 mRNA and mtATP6 in myocardial tissue. One-way ANOVA were performed in multigroup mean comparison, LSD-t test was used to compare the mean values of two samples, and P<0.05 was considered statistically significant. Results The pathological score of myocardial tissue in the Cur group was lower than those in the CLP group at 6 h and 24 h (P 0.05). Conclusion Curcumin protects acute myocardial injury by affecting HMGB1 release and translocation down-regulation of extracellular levels and regulation of mtATP6 expression in CLP model rats. Key words: Sepsis; Myocardial injury; High mobility group box 1; Mitochondrial gene; Curcumin; Rat

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姜黄素对脓毒症心肌损伤大鼠线粒体基因及高迁移率组盒1蛋白的影响

目的观察姜黄素对脓毒症大鼠心肌线粒体相关基因（mtATP6）、高迁移率组蛋白1（HMGB1）mRNA表达和血浆HMGB1水平的影响，探讨保护脓毒症心肌损伤的新策略。方法采用盲肠结扎穿刺法建立大鼠败血症模型。将30只雄性Sprague-Dawley大鼠随机分为假手术组（假手术组）、败血症组（CLP组）和姜黄素干预组（Cur组），每组10只。Cur组大鼠腹腔注射姜黄素100mg/kg，假手术组和CLP组大鼠注射生理盐水。在建模后6小时和24小时随机（随机数）处死5只大鼠。光镜下观察心肌组织的病理变化。检测血清CK和CK-MB水平。ELISA法检测血浆HMGB1水平。采用实时聚合酶链式反应检测心肌组织中HMGB1 mRNA和mtATP6的表达。单因素方差分析用于多组平均值比较，LSD-t检验用于比较两个样本的平均值，P<0.05被认为具有统计学意义。结果姜黄素组心肌组织病理学评分在6h和24h均低于CLP组（P＜0.05）。关键词：败血症；心肌损伤；高移动性组盒1；线粒体基因；姜黄素；大鼠

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来源期刊

中华急诊医学杂志 Nursing-Emergency Nursing

CiteScore

0.10

自引率

0.00%

发文量

8629

期刊介绍： Chinese Journal of Emergency Medicine is the only national journal which represents the development of emergency medicine in China. The journal is supervised by China Association of Science and Technology, sponsored by Chinese Medical Association, and co-sponsored by Zhejiang University. The journal publishes original research articles dealing with all aspects of clinical practice and research in emergency medicine. The columns include Pre-Hospital Rescue, Emergency Care, Trauma, Resuscitation, Poisoning, Disaster Medicine, Continuing Education, etc. It has a wide coverage in China, and builds up communication with Hong Kong, Macao, Taiwan and international emergency medicine circles.