Effects of hyperbaric oxygen on the expression of osteoprotegerin and integrin β1 in rabbit＇s mandibular distraction gap

中华医学美学美容杂志 Pub Date : 2019-06-15 DOI:10.3760/CMA.J.ISSN.1671-0290.2019.03.002

W. Shangguan, Chun-bing Hu, Hao Dai, Shu Wang

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Abstract

Objective To investigate the effect of hyperbaric-oxygen therapy on osteoprotegerin (OPG) and integrin β1 (Itgβ1) expression during mandibular distraction osteogenesis. Methods Forty New-Zealand rabbits were used. The animals were randomly divided into two groups (experimental group and control group) with 20 animals each; after accomplished osteotomy and implant distraction devices on mandible bilaterally for 3 days of latency period, the device was activated at the rate of 0.8 mm per day for 10 days. All animals in the experimental group were subjected to hyperbaric oxygen for 90 minutes once a day since the beginning of distraction, and lasted for four weeks. In control group, all the distractors were activated following the same distraction protocol as the experimental group, but without hyperbaric oxygen therapy. Five animals of each group were sacrificed at 10th day after distraction, 7th, 14th and 28th day of consolidation, respectively. The lengthened mandibles were harvested and processed for immunohistochemical examinations to detect OPG and Itgβ1 expression in the distraction gap. Semi-quantitative analysis was carried out by image analysis software. Results OPG staining was mainly located in the membrane and cytoplasm of osteoprogenitor cells and osteoblasts in the distraction zones. The expression of OPG increased after distraction accomplished and reached to the peak at 7th day of consolidation, and then decreased gradually. At every time point, the level of expression of OPG in the experimental group was remarkably higher than those in control group. There were significant differences between the experimental group and control group (P 0.05). Conclusions Hyperbaric oxygen therapy can up-regulate the expression of OPG and Itgβ1 in the distraction gap, which may promote osteoblast differentiation, proliferation, enhance osteoblast function, and new bone formation in distraction gap. Key words: Rabbits; Traction; Mandible; Integrin beta1; Osteoprotegerin; Hyperbaric-oxygen

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高压氧对兔下颌牵张间隙骨保护素和整合素β1表达的影响

目的探讨高压氧治疗对下颌骨牵张成骨过程中骨保护蛋白（OPG）和整合素β1（Itgβ1）表达的影响。方法选用新西兰兔40只。将动物随机分为两组（实验组和对照组），每组20只；在双侧下颌骨截骨和种植体牵引装置完成3天的潜伏期后，该装置以每天0.8mm的速率激活10天。实验组的所有动物自分心开始以来每天接受一次高压氧90分钟，持续四周。对照组按照与实验组相同的牵张方案激活所有牵张器，但不进行高压氧治疗。每组5只动物分别在分散后第10天、巩固后第7天、第14天和第28天处死。采集延长的下颌骨并进行免疫组织化学检查，以检测牵引间隙中OPG和Itgβ1的表达。采用图像分析软件进行半定量分析。结果OPG染色主要位于牵张区的骨祖细胞和成骨细胞的膜和细胞质中。OPG的表达在牵张完成后增加，并在巩固第7天达到峰值，然后逐渐下降。在每个时间点，实验组的OPG表达水平都显著高于对照组。结论高压氧治疗可上调牵张间隙OPG和Itgβ1的表达，促进成骨细胞分化、增殖，增强成骨细胞功能，促进牵张间隙新骨形成。关键词：兔子；牵引；下颌骨；整合素β1；骨保护素；高压氧

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来源期刊

中华医学美学美容杂志

自引率

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发文量

4641

期刊介绍： "Chinese Journal of Medical Aesthetics and Cosmetology" is a high-end academic journal focusing on the basic theoretical research and clinical application of medical aesthetics and cosmetology. In March 2002, it was included in the statistical source journals of Chinese scientific and technological papers of the Ministry of Science and Technology, and has been included in the full-text retrieval system of "China Journal Network", "Chinese Academic Journals (CD-ROM Edition)" and "China Academic Journals Comprehensive Evaluation Database". Publishes research and applications in cosmetic surgery, cosmetic dermatology, cosmetic dentistry, cosmetic internal medicine, physical cosmetology, drug cosmetology, traditional Chinese medicine cosmetology and beauty care. Columns include: clinical treatises, experimental research, medical aesthetics, experience summaries, case reports, technological innovations, reviews, lectures, etc.