Inhibitory effect of standardized extract and fractions of Nigella sativa L. on nystatin susceptible and clinically nystatin resistant Candida albicans.

Q3 Pharmacology, Toxicology and Pharmaceutics

Current drug discovery technologies Pub Date : 2022-05-12 DOI:10.2174/1570163819666220512164337

Abolfazl Saravani Jahantiq, S. A. Ayatollahi Mousavi, N. Mohamadi, F. Sharififar

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Abstract

INTRODUCTION Candidiasis infection is caused by different species of Candida, which are characterized by host immunologic weakness. Black cumin seeds (Nigella sativa) have shown inhibitory effect against Candida albicans. In this work, the inhibitory effect of standardized extract and different fractions of Nigella sativa seeds has been evaluated on nystatin susceptible. MATERIALS AND METHOD Canadida albicans (NSCA) with ATCC 76645 and nystatin resistant Candida albicans (NRCA) was prepared from oral samples of HIV individuals. Total extract and different fractions of N. sativa were prepared using maceration and sonication methods. Thymoquinone (TQ) content of the plant was determined by spectrophotometry. Total extract (TTE) and the fractions along with TQ were evaluated on NSCA and NRCA by microdilution method. TQ content of the plant was 0.92±0.37g/100g dried extract. The least MIC and MFC (62.5 and 125 µg/ml respectively) was due to petroleum ether fraction (PEF) against both NSCA and NRCA followed by chloroform fraction (CHF) with MIC and MFC of 125 and 250 µg/ml. TQ exhibited MIC of 0.78 and 3.12 µg/ml against NSCA and NRCA which was stronger than nystatin (MIC of 2 and 16 µg/ml). Results Thymoquinone was detected in the PEF and CHF. CONCLUSION Considering more inhibitory effects of PEF and CHF than TTE, can conclude that active components of the plant belong to non-polar compounds. PEF showed identical inhibitory effect on NRCA and NSCA that is valuable result for finding novel medicaments against NRCA infections.

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Nigella sativa L.标准化提取物和级分对制霉菌素敏感和临床抗制霉菌素白色念珠菌的抑制作用。

念珠菌感染是由不同种类的念珠菌引起的，其特征是宿主免疫无力。黑孜然种子（Nigella sativa）对白色念珠菌具有抑制作用。在这项工作中，已经评估了奈杰尔拉种子的标准化提取物和不同组分对制霉菌素敏感的抑制作用。材料和方法从HIV个体的口腔样本中制备具有ATCC 76645的白色念珠菌（NSCA）和制霉菌素抗性白色念珠菌（NRCA）。采用浸渍和超声处理的方法制备了N.sativa的总提取物和不同组分。采用分光光度法测定了该植物中胸腺醌（TQ）的含量。通过微量稀释法在NSCA和NRCA上评估总提取物（TTE）和组分以及TQ。该植物的TQ含量为0.92±0.37g/100g干提取物。MIC和MFC最低（分别为62.5和125µg/ml）是由于石油醚组分（PEF）对NSCA和NRCA的作用，其次是氯仿组分（CHF），MIC和MFC分别为125和250µg/ml。TQ对NSCA和NRCA的MIC分别为0.78和3.12µg/ml，强于制霉菌素（MIC为2和16µg/ml）。结果在PEF和CHF中均检出胸腺醌。结论考虑到PEF和CHF的抑制作用大于TTE，可以得出该植物的活性成分属于非极性化合物的结论。PEF对NRCA和NSCA表现出相同的抑制作用，这对于寻找新的治疗NRCA感染的药物是有价值的结果。

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来源期刊

Current drug discovery technologies Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

3.70

自引率

0.00%

发文量

期刊介绍： Due to the plethora of new approaches being used in modern drug discovery by the pharmaceutical industry, Current Drug Discovery Technologies has been established to provide comprehensive overviews of all the major modern techniques and technologies used in drug design and discovery. The journal is the forum for publishing both original research papers and reviews describing novel approaches and cutting edge technologies used in all stages of drug discovery. The journal addresses the multidimensional challenges of drug discovery science including integration issues of the drug discovery process.