Effect of low intensity pulsed ultrasound on osteoclast differentiation

IF 0.5 Q4 DENTISTRY, ORAL SURGERY & MEDICINE

Orthodontic Waves Pub Date : 2020-11-06 DOI:10.1080/13440241.2020.1843354

Yuri Higashi, K. Inami, H. Shimizu, N. Matsumoto

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引用次数: 1

Abstract

ABSTRACT Purpose For many years, people have attempted to identify ways of efficiently moving teeth in orthodontic therapy to reduce harmful effects. In this study, we investigate whether the non-surgical method of low-intensity pulsed ultrasound (LIPUS) irradiation affects osteoclast differentiation in vitro. Materials and Methods RAW264 cells were stimulated with RANKL and U0126, and LIPUS irradiation was performed using different ultrasonic outputs, irradiation times, and number of irradiations. Assessments used TRAP staining, TRAP quantitative assays, and western blot analysis. Results LIPUS irradiation significantly increased osteoclast differentiation rates of RAW264 cells. The rates were significantly higher in the three-time irradiation group compared with the single irradiation group. However, significant differences were not observed between the ultrasonic output. TRAP quantitative assay results showed significantly increased osteoclast differentiation rates with increased irradiation time, however, only in the single irradiation group. Conclusion These results suggest that an increased number of LIPUS irradiations may improve its effectiveness in orthodontic therapy.

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低强度脉冲超声对破骨细胞分化的影响

摘要:目的多年来，人们一直试图在正畸治疗中寻找有效移动牙齿的方法，以减少有害影响。在这项研究中，我们研究了非手术方法低强度脉冲超声(LIPUS)照射是否影响体外破骨细胞分化。材料和方法以RANKL和U0126刺激RAW264细胞，并采用不同的超声输出、照射次数和照射次数进行LIPUS照射。评估采用TRAP染色、TRAP定量分析和western blot分析。结果LIPUS辐照显著提高RAW264细胞的破骨细胞分化率。三次照射组的发生率明显高于单次照射组。然而，在超声输出之间没有观察到显著差异。TRAP定量分析结果显示，随着照射时间的增加，破骨细胞分化率显著增加，但仅在单次照射组。结论增加LIPUS照射次数可提高其在正畸治疗中的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Orthodontic Waves DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

0.40

自引率

0.00%

发文量

期刊介绍： Orthodontic Waves is the official publication of the Japanese Orthodontic Society. The aim of this journal is to foster the advancement of orthodontic research and practice. The journal seeks to publish original articles (i) definitive reports of wide interest to the orthodontic community, (ii) Case Reports and (iii) Short Communications. Research papers stand on the scientific basis of orthodontics. Clinical topics covered include all techniques and approaches to treatment planning. All submissions are subject to peer review.