ANTI-DIABETIC STUDY AND ITS QUANTITATIVE REAL-TIME PCR ANALYSIS OF THE INSR GENE IN SILVERNANOPARTICLE EXTRACT OF CHAMAECOSTUS CUSPIDATUS

Abstract

Manufacturing metal oxide nanoparticles for chemical and biomedical applications is made possible by green synthesis, an eco-friendly approach. This investigation examined how silver nanoparticle leaf extract affected gene expression and its ability to treat diabetes. In this study, the Anti-diabetic study was evaluated by following the Glucose utilization control method. Furthermore, the study continues with Quantitative Real Time-PCR Analysis of the INSR Gene. The analysis's findings showed that, when used as a control, metformin exhibits a glucose percentage of 169.26, whereas silver nanoparticle extracts exhibit a percentage control of 169.53. HepG2 cells treated with AgNPs C.cuspidtus showed upregulated INSR gene expression, going from 1-fold in control cells to 1.51-fold in treated cells. The relative gene expression of the control is typically set to 1 when compared to the sample. The study shows that glucose absorption in AgNPs extract is slightly higher than in methanolic extract. As a result, there is a pressing mandate to protect this species and investigate its biological applications to achieve more significant and worthwhile outcomes.