Amal D. Premarathna , T.H. Ranahewa , S.K. Wijesekera , R.R.M.K.K. Wijesundara , Anura P. Jayasooriya , Viskam Wijewardana , R.P.V.J. Rajapakse
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引用次数: 19
Abstract
Background
Seaweeds contain biologically active molecules which are important in the pharmaceutical industry. The present study was aimed to investigate the effects of aqueous extract of seaweeds:Sargassum illicifolium (S.i) on the stimulation of mouse fibroblast cell proliferation and migration.
Materials and methods
Sargassum illicifolium-SW12, Sargassum illicifolium-SW13, and Sargassum illicifolium-SW23 were collected from Northern, North Western and Southern coastal sites of Sri Lanka respectively. To determine the cytotoxic of S.i extracts were used the MTT assay. The scratch wound healing assay was performed using the mouse fibroblasts (L929) cells that assess the cell proliferation and migration. The highest cell proliferation of S.i species aqueous extracts was measured by observing the change in the size of the scratch wound area.
Results
A concentration of S.i extracts 0.954 ± 0.04 μg/μl (Mean ± SE), (P < 0.05) had highest proliferative and migratory effect on L929 cells when compared with the control. Cell proliferation and/or migration was as follows: S.i-SW23 extracts (0.97 μg/μl, 205.4 ± 17.32%) was higher compared to extracts of S.i-SW12 (1.06 μg/μl, 175.8 ± 13.09%), S.i-SW13 (0.94 μg/μl, 178.8 ± 2.22%) and the control (100%). Scratch wound healing of S.i-SW23 extracts exhibited a significant enhancement within 24 h (P < 0.05) with a wound area of 97.83 ± 0.05% compared with the control (46.11 ± 0.54%). At 24 h S.i-SW23 extracts completely closed the wound.
Conclusions
Cell proliferation and migration were significantly faster in those treated with S.i-SW23 aqueous extracts when compared with the control group. Moreover, no cytotoxic effect of S.i aqueous extracts on L929 cell line were observed. This study suggests that S.i might be a potential therapeutic agent for skin wound healing by promoting fibroblast proliferation and migration. Further studies are highly warranted to identify active molecules in the extracts. Moreover, in-vivo studies would further verify wound healing therapeutic applications shown here.
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