Antibiotic Resistance and Biofilm Production in Catalase-Positive Gram-Positive Cocci Isolated from Brazilian Pasteurized Milk

Q3 Agricultural and Biological Sciences

Journal of Food Quality and Hazards Control Pub Date : 2020-05-30 DOI:10.18502/jfqhc.7.2.2886

M.A.A. Machado, W. A. Ribeiro, V. Toledo, G. L. Ramos, H. C. Vigoder, J. S. Nascimento

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引用次数: 8

Abstract

Background: Milk is a reservoir for several groups of microorganisms, which may pose health risks. The aim of this work was to assess the antibiotic resistance and biofilm production in catalase-positive Gram-positive cocci isolated from Brazilian pasteurized milk. Methods: The bacteria were isolated using Baird-Parker agar and identified by MatrixAssisted Laser Desorption/Ionization-Time-Of-Flight (MALDI-TOF) mass spectrometer. Disk diffusion technique was used to evaluate antimicrobial susceptibility. For qualitative evaluation of biofilm production, the growth technique was used on Congo Red Agar. Results: Totally, 33 out of 64 isolates were identified, including Staphylococcus epidermidis (n=3; 4.7%), Macrococcus caseolyticus (n=14; 21.9%), and Kocuria varians (n=16; 25%). Twenty-two isolates were resistant to at least one antibiotic. Biofilm production was detected in only 5 isolates of K. varians and 1 isolate of S. epidermidis. All 14 M. caseolyticus isolates were resistant to at least one antibiotic; but, multidrug resistant (MDR) isolates were not detected. Among all K. varians isolates, 4 were resistant to at least one antibiotic from three different classes and were considered to be MDR. Conclusion: The presence of antibiotic-resistant M. caseolyticus, S. epidermidis, and K. varians isolates, especially MDRs, in milk samples highlights the possible role of milk as a reservoir of resistance genes

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巴西巴氏奶中过氧化氢酶阳性革兰氏阳性球菌的抗生素耐药性和生物膜生成

背景:牛奶是几组微生物的储存库，可能对健康构成威胁。这项工作的目的是评估从巴西巴氏奶中分离的过氧化氢酶阳性革兰氏阳性球菌的抗生素耐药性和生物膜的产生。方法:采用Baird-Parker琼脂法分离细菌，采用基质辅助激光解吸/电离飞行时间(MALDI-TOF)质谱仪进行鉴定。采用纸片扩散法进行药敏评价。为了对生物膜的生成进行定性评价，在刚果红琼脂上采用了生长技术。结果:64株分离物中检出33株，其中表皮葡萄球菌(n=3;4.7%)，溶干酪巨球菌(n=14;21.9%)和Kocuria变种(n=16;25%)。22株菌株对至少一种抗生素具有耐药性。仅有5株变异克雷伯氏菌和1株表皮葡萄球菌分离株检测到生物膜生成。所有14株溶干酪分枝杆菌分离株均对至少一种抗生素耐药;但未检出耐多药(MDR)菌株。在所有分离株中，有4株对来自三种不同类别的至少一种抗生素耐药，被认为是耐多药。结论:牛奶样品中存在耐药溶干酪分枝杆菌、表皮葡萄球菌和变异克雷伯菌分离株，特别是耐多药葡萄球菌，这表明牛奶可能是耐药基因的储存库

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来源期刊

Journal of Food Quality and Hazards Control Agricultural and Biological Sciences-Food Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of Food Quality and Hazards Control (J. Food Qual. Hazards Control) is an international peer-reviewed quarterly journal that aims at publishing of high quality articles involved in food quality, food hygiene, food safety, and food control which scientists from all over the world may submit their manuscript. This academic journal aims to improve international exchange of new findings and recent developments in all aspects of agricultural and biological sciences. This free of charge journal is published in both online and print forms and welcomes the manuscripts that fulfill the general criteria of novelty and scientific importance. Among the most significant objectives of Journal of Food Quality and Hazards Control are to ensure that the articles reflect a wide range of topics regarding journal scopes; to do a fair, scientific, fast, as well as high quality peer-review process; to provide a wide and diverse geographical coverage of articles around the world; and to publish the articles having a trustable resource of scientific information for the audiences. The types of acceptable submissions include original article, review article, short communication, letter to the editor, case report, editorial, as well as book review. Journal of Food Quality and Hazards Control is an official journal of Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.