Gas Chromatography with Modified pH‐sensitive Pellets in Evaluating the Activity of Some Enzymes Attributed with a Change in the Medium pH

Q3 Pharmacology, Toxicology and Pharmaceutics

Jordan Journal of Pharmaceutical Sciences Pub Date : 2023-07-24 DOI:10.35516/jjps.v16i2.1484

Muhammed Alzweiri

{"title":"Gas Chromatography with Modified pH‐sensitive Pellets in Evaluating the Activity of Some Enzymes Attributed with a Change in the Medium pH","authors":"Muhammed Alzweiri","doi":"10.35516/jjps.v16i2.1484","DOIUrl":null,"url":null,"abstract":"A new evaluation method based on gas chromatography (GC) is considered for the screening of the inhibitory activity of various compounds against certain enzymes using immobilized pH-sensitive matrix pellets. This approach of analysis is being utilized in developing sensitive GC methods for enzymes attributed to pH change in their media. Particularly, for those which require accurate and sensitive assay methods. The method has been implemented in drug discovery research related to antagonism of carbonic anhydrase, isozyme 3 (CAIII). This isozyme has a strong potential in treating obesity, hyperlipidemia and cancer. The method was also used in investigation of various peptide derivatives with an epoxide warhead for their anti-lipase activity. Recently, the method has been used in evaluating some tacrine analogues for their inhibition against acetylcholinesterase enzyme. The latter is important in treating Alzheimer’s disease (AD) which is a worldwide mental disorder manifested with dementia symptoms and anticipated to be the second causality of deaths by 2040.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jordan Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35516/jjps.v16i2.1484","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}

引用次数: 0

Abstract

A new evaluation method based on gas chromatography (GC) is considered for the screening of the inhibitory activity of various compounds against certain enzymes using immobilized pH-sensitive matrix pellets. This approach of analysis is being utilized in developing sensitive GC methods for enzymes attributed to pH change in their media. Particularly, for those which require accurate and sensitive assay methods. The method has been implemented in drug discovery research related to antagonism of carbonic anhydrase, isozyme 3 (CAIII). This isozyme has a strong potential in treating obesity, hyperlipidemia and cancer. The method was also used in investigation of various peptide derivatives with an epoxide warhead for their anti-lipase activity. Recently, the method has been used in evaluating some tacrine analogues for their inhibition against acetylcholinesterase enzyme. The latter is important in treating Alzheimer’s disease (AD) which is a worldwide mental disorder manifested with dementia symptoms and anticipated to be the second causality of deaths by 2040.

查看原文本刊更多论文

气相色谱法测定pH敏感微球对某些酶活性的影响

考虑了一种基于气相色谱（GC）的新评估方法，用于使用固定化pH敏感基质颗粒筛选各种化合物对某些酶的抑制活性。这种分析方法正被用于开发敏感的GC方法，用于分析介质中pH变化引起的酶。特别是对于那些需要准确和灵敏的测定方法的人。该方法已应用于与碳酸酐酶同工酶3（CAII）拮抗作用相关的药物发现研究中。这种同功酶在治疗肥胖、高脂血症和癌症方面具有很强的潜力。该方法还用于研究具有环氧弹头的各种肽衍生物的抗脂肪酶活性。最近，该方法已被用于评估一些他克林类似物对乙酰胆碱酯酶的抑制作用。后者在治疗阿尔茨海默病（AD）方面很重要，阿尔茨海默病是一种全球性的精神障碍，表现为痴呆症状，预计到2040年将成为死亡的第二个原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Jordan Journal of Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： The Jordan Journal of Pharmaceutical Sciences (JJPS) is a scientific, bi-annual, peer-reviewed publication that will focus on current topics of interest to the pharmaceutical community at large. Although the JJPS is intended to be of interest to pharmaceutical scientists, other healthy workers, and manufacturing processors will also find it most interesting and informative. Papers will cover basic pharmaceutical and applied research, scientific commentaries, as well as views, reviews. Topics on products will include manufacturing process, quality control, pharmaceutical engineering, pharmaceutical technology, and philosophies on all aspects of pharmaceutical sciences. The editorial advisory board would like to place an emphasis on new and innovative methods, technologies, and techniques for the pharmaceutical industry. The reader will find a broad range of important topics in this first issue.