Synergistic effect of certain insecticides combined with Bacillus thuringiensis on mosquito larvae

Q3 Agricultural and Biological Sciences

Journal of Entomological and Acarological Research Pub Date : 2017-04-21 DOI:10.4081/JEAR.2017.6265

Chandrakant P. Narkhede, C. Patil, R. Suryawanshi, Sunil H. Koli, B. V. Mohite, S. Patil

{"title":"Synergistic effect of certain insecticides combined with Bacillus thuringiensis on mosquito larvae","authors":"Chandrakant P. Narkhede, C. Patil, R. Suryawanshi, Sunil H. Koli, B. V. Mohite, S. Patil","doi":"10.4081/JEAR.2017.6265","DOIUrl":null,"url":null,"abstract":"For effective vector control it is essential to formulate new preparations having multiple action against the vector pest. Developing combined formulation of biopesticide and chemical pesticide is one of the novel concept to fight against the vectors with new weapons; however, compatibility of biopesticide i.e . Bacillus thuringiensis (Bt) and chemical pesticide is a real hurdle. In this investigation, local isolate Bacillus thuringiensis SV2 (BtSV2) was tested for its compatibility with various available mosquito larvicides. Temephos was most compatible with BtSV2 than with other tested pesticides. These two compatible agents were tested for larvicidal potential. Our study revealed that the synergistic effect of both agents reduces LC50 value by 30.68 and 22.36% against the Ae . aegypti and An . stephensi , respectively. The larvicidal potential increased when compared to individual pesticides. It was also observed a biochemical change in larvae after the TBT (Temephos + Bacillus thuringiensis) combination treatment; it involves decreased level of alpha esterase, acetylcholine esterase and protein while level of beta esterase and acid phosphatase was unchanged and alkaline phosphatase activity was increased. Increased potential of combined formulation may be due to altered physiological condition.","PeriodicalId":37777,"journal":{"name":"Journal of Entomological and Acarological Research","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2017-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4081/JEAR.2017.6265","citationCount":"6","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Entomological and Acarological Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4081/JEAR.2017.6265","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 6

Abstract

For effective vector control it is essential to formulate new preparations having multiple action against the vector pest. Developing combined formulation of biopesticide and chemical pesticide is one of the novel concept to fight against the vectors with new weapons; however, compatibility of biopesticide i.e . Bacillus thuringiensis (Bt) and chemical pesticide is a real hurdle. In this investigation, local isolate Bacillus thuringiensis SV2 (BtSV2) was tested for its compatibility with various available mosquito larvicides. Temephos was most compatible with BtSV2 than with other tested pesticides. These two compatible agents were tested for larvicidal potential. Our study revealed that the synergistic effect of both agents reduces LC50 value by 30.68 and 22.36% against the Ae . aegypti and An . stephensi , respectively. The larvicidal potential increased when compared to individual pesticides. It was also observed a biochemical change in larvae after the TBT (Temephos + Bacillus thuringiensis) combination treatment; it involves decreased level of alpha esterase, acetylcholine esterase and protein while level of beta esterase and acid phosphatase was unchanged and alkaline phosphatase activity was increased. Increased potential of combined formulation may be due to altered physiological condition.

查看原文本刊更多论文

某些杀虫剂与苏云金芽孢杆菌联合对蚊虫幼虫的增效作用

为有效控制病媒，必须研制对病媒害虫具有多重作用的新制剂。开发生物农药与化学农药联合制剂是利用新武器对抗病媒生物的新思路之一;然而，生物农药的相容性，即苏云金芽孢杆菌(Bt)和化学农药是一个真正的障碍。本研究对苏云金芽孢杆菌本地分离株SV2 (BtSV2)与多种杀虫剂的配伍性进行了检测。双硫磷与BtSV2的相容性较好。试验了两种配伍剂的杀虫效果。研究结果表明，两种药剂的协同作用使其对伊蚊的LC50值分别降低30.68%和22.36%。埃及伊蚊和安。Stephensi，分别。与单个杀虫剂相比，其杀虫潜力有所增加。双硫磷+苏云金芽孢杆菌(TBT)联合处理后，幼虫的生化变化明显;α -酯酶、乙酰胆碱酯酶和蛋白质水平降低，β -酯酶和酸性磷酸酶水平不变，碱性磷酸酶活性升高。联合制剂的潜力增加可能是由于生理条件的改变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Entomological and Acarological Research Agricultural and Biological Sciences-Insect Science

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Entomological and Acarological Research (JEAR), formerly the Bollettino di Zoologia Agraria e di Bachicoltura of the Institute of Entomology of the Università degli Studi, Milano, was founded in 1928 by Remo Grandori. Thereafter, Minos Martelli and Luciano Süss hold the direction of the Journal until December 2011. In January 2012 the Editor decided for the new open-access on-line version of JEAR. The Journal publishes original research papers concerning Arthopods, but reviews, editorials, technical reports, brief notes, conference proceeding, letters to the Editor, book reviews are also welcome. JEAR has four main areas of interest: -Entomology (systematics; morphology; biology; biotechnology; agriculture, ornamental and forest entomology; applied entomology; integrated pest management; biological control; apiculture and apidology; medical, urban and veterinary entomology; etc.) -Stored product pests (biology; integrated pest management; etc.) -Insect Ecology (behaviour; biodiversity; taxonomy; plant insect interaction and ecosystems; biological control; alien species; etc.) -Acarology (systematics; morphology; biology; parasitology; control; etc.) The publication of manuscripts is subject to the approval of the Section Editor who has knowledge of the field discussed in the manuscript in accordance with the principles of Peer Review; referees will be selected from the Editorial Board or among qualified scientists of the international scientific community. Articles must be written in English and must adhere to the guidelines and details contained in the Instructions to Authors.