IMPROVEMENT OF OMEGA-3 CONTENT OF BLACK SOLDIER FLY PREPUPA (Hermetia illucens) FED WITH COMBINATION OF MARINE FISH OFFAL AND TOFU DREG

Q3 Agricultural and Biological Sciences

Biotropia Pub Date : 2021-04-29 DOI:10.11598/BTB.0.0.0.1082

R. E. Putra, Yenyen Fatmalasari, A. Permana, I. Kinasih, M. Rosmiati

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Abstract

One of the materials with great potency for future nutrition source for animal feed is prepupae of black soldier fly larvae (BSFP) (Hermetia illucens) which is fed on organic wastes. This study was designed to observe the accumulation of specific substance (omega-3) of organic wastes (marine fish offal and tofu dregs) inside harvested biomass of BSFP. A total of 150 gram food consist of marine fish offal and tofu dreg with different proportion (10:90, 25:75, 50:50, and 100% tofu dregs) was fed to black soldier fly larvae (BSFL) for 21 days.At the end of feeding trial, all BSFL were harvested, weighed, and then analyzed for omega-3 fatty acids content. Fatty acid content was analyzed using Gas Cromatography with Flame Ionization Detector (GC-FID). Result of the experiment showed BSFL reared with 25% marine fish offal produced highest biomass (8.1 g / 50 larvae) with least development time (19 days). The total of omega-3 recorded from application of 0%, 10%, 25%, and 50% of marine fish offal was 0.02%, 0.87%, 2.16% and 2.61% in 100 g of dry weight, respectively. This result showed the possibility of transferring specific nutrient from organic wastes biomass of BSFP which provides base knowledgement for further application in design of specific animal feed from BSFP.

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海鱼内脏与豆腐渣复合饲料对黑虻预蛹OMEGA-3含量的提高

黑蝇幼虫（BSFP）是以有机废物为原料制备的，是未来动物饲料营养来源的重要材料之一。本研究旨在观察有机废物（海鱼内脏和豆腐渣）中特定物质（ω-3）在BSFP收获的生物质中的积累。将150克海鱼内脏和豆腐渣按不同比例（10∶90、25∶75、50∶50和100%豆腐渣）喂给黑蝇幼虫21天。在饲养试验结束时，采集所有BSFL，称重，然后分析ω-3脂肪酸含量。脂肪酸含量采用火焰离子化检测器（GC-FID）气相色谱法进行分析。实验结果表明，用25%的海鱼内脏饲养BSFL，其生物量最高（8.1g/50幼虫），发育时间最少（19天）。在100g干重中，施用0%、10%、25%和50%的海鱼内脏记录的ω-3总量分别为0.02%、0.87%、2.16%和2.61%。这一结果表明了从BSFP的有机废弃物生物质中转移特定营养素的可能性，为BSFP在特定动物饲料设计中的进一步应用提供了基础知识。

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