Expression and clinical significance of microRNA-126, microRNA-155, Klotho protein and TGF-β1 protein in peripheral blood of patients with essential hypertension

中国基层医药 Pub Date : 2020-04-01 DOI:10.3760/CMA.J.ISSN.1008-6706.2020.07.007

Junchong Chen

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引用次数: 0

Abstract

Objective To investigate the expression of microRNA-126, microRNA-155, Klotho protein and transforming growth factor-β1 (TGF-β1) in peripheral blood of patients with essential hypertension. Methods From December 2015 to December 2018, 80 patients with essential hypertension admitted to Lishui Central Hospital were selected in the study, and 80 healthy people from Lishui Central Hospital from December 2015 to December 2018 were selected as the control group.The expressions of microRNA-126 and microRNA-155 were detected by RT-PCR, and the expressions of Klotho and TGF-β1 were detected by ELISA.The changes of systolic and diastolic blood pressure, the expressions of microRNA-126 and microRNA-155 in peripheral blood, Klotho protein and TGF-β1 protein were compared between the two groups. Results The expression of microRNA-126 (0.23±0.07) and microRNA-155 (0.84±0.14) in peripheral blood of the hypertension group was lower than that of the control group [(1.16±0.24) and (1.37±0.21)] (t=33.273, 18.782, all P<0.05). The serum levels of Klotho protein in the hypertension group[(123.42±9.47)ng/L] was lower than that in the control group [(143.56±14.68)ng/L], while the serum level of TGF-β1 protein the hypertension group[(561.32±58.39)ng/L] was higher than that in the control group [(408.97±25.13)ng/L] (t=10.312, 21.436, allP<0.05). Conclusion The expression of microRNA-126 and microRNA-155 in peripheral blood of patients with essential hypertension is down-regulated, and the expression of Klotho protein and TGF-β1 protein is up-regulated, which has certain guiding significance for clinical diagnosis and treatment. Key words: Hypertension; MicroRNA-126; MicroRNA-155; Klotho protein; Transforming growth factor β1

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原发性高血压患者外周血中microRNA-126、microRNA-155、Klotho蛋白、TGF-β1蛋白的表达及临床意义

目的探讨原发性高血压患者外周血微小核糖核酸-126、微小核糖核酸-155、Klotho蛋白和转化生长因子-β1（TGF-β1）的表达。方法选取丽水市中心医院2015年12月至2018年12月收治的80例原发性高血压患者作为研究对象，丽水市中心医院15年12月～2018年12月份收治的80名健康人作为对照组。用RT-PCR法检测microRNA-126和microRNA-155的表达，用ELISA法检测Klotho和TGF-。结果高血压组外周血微小RNA-126（0.23±0.07）和微小RNA-155（0.84±0.14）的表达低于对照组[（1.16±0.24）和（1.37±0.21）]（t=33.27318.782，均P<0.05），而高血压组血清TGF-β1蛋白水平[（561.32±58.39）ng/L]高于对照组[（408.97±25.13）ng[L]（t=10.312，21.436，allP<0.05），对临床诊断和治疗具有一定的指导意义。关键词：高血压；MicroRNA-126；微小RNA-155；Klotho蛋白；转化生长因子β1

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来源期刊

中国基层医药

CiteScore

0.10

自引率

0.00%

发文量

32251

期刊介绍： Since its inception, the journal "Chinese Primary Medicine" has adhered to the development strategy of "based in China, serving the grassroots, and facing the world" as its publishing concept, reporting a large amount of the latest medical information at home and abroad, prospering the academic field of primary medicine, and is praised by readers as a medical encyclopedia that updates knowledge. It is a core journal in China's medical and health field, and its influence index (CI) ranks Q2 in China's academic journals in 2022. It was included in the American Chemical Abstracts in 2008, the World Health Organization Western Pacific Regional Medical Index (WPRIM) in 2009, and the Japan Science and Technology Agency Database (JST) and Scopus Database in 2018, and was included in the Wanfang Data-China Digital Journal Group and the China Academic Journal Comprehensive Evaluation Database.