STUDY OF THE EFFECT OF NEW 6-R-3,5-DINITRO-1,2,3,4-TETRAHYDROPYRIDINES ON THE ACCUMULATION OF PHOTOSYNTHETIC PIGMENTS AND ASCORBIC ACID IN PLANT TISSUES

Abstract

Background. The problem of finding new effective plant protection products remains an urgent task of modern agrochemistry. It is known that tetrahydropyridine derivatives exhibit a broad spectrum of biological activity, including fungicidal activity. However, compounds with potential biological activity can also be highly toxic to humans, cultivated crops, and the environment as a whole, so it is necessary to study their environmental safety. The content of photosynthetic pigments and ascorbic acid are one of the most important biochemical indicators of plant response to environmental stress factors, therefore, determining their amount helps to assessthe physiological state of plants and analyze the toxicity of the synthesized compounds. Purpose. To study the effect of new 6-R-3,5-dinitro-1,2,3,4-tetrahydropyridines on the content of photosynthetic pigments and ascorbic acid in plant tissues in order to assess the safety of their use as potential plant protection products. Materials and methods. The test compounds were obtained by the previously developed method for the selective reduction of 2-R-3,5-dinitropyridines. Winter wheat seeds of the Moskovskaya 39 variety were germinated in Petri dishes using solutions of the synthesized substances. Seeds germinated in water were used as controls. On the ninth day, the content of various groups of photosynthetic pigments (chlorophylls a, b, carotenoids), as well as ascorbic acid in plant tissues was determined. All experiments were carried out in triplicate. In addition, the analytes were tested for toxicity using the GUSAR Environmental Toxicity software. Results. The effect of new derivatives of tetrahydropyridine on the accumulation of photosynthetic pigments and ascorbic acid in plant tissues of winter wheat was studied. It was shown that the studied compounds do not have a significant toxic effect on plants. Conclusion. As a result of studying the effect of new 6-R-3,5-dinitro-1,2,3,4-tetrahydropyridines on the content of photosynthetic pigments and ascorbic acid, as well as in silico computer prediction in GUSAR Environmental Toxicity, it was found that, in general, the studied compounds are relatively safe and, therefore, promising for further testing as biologically active plant protection products.