Phenotypic and transcriptomic characterization of an ABA-sensitive mutant generated by microspore embryogenesis in barley

IF 2.6 3区生物学 Q2 PLANT SCIENCES

Journal of Plant Interactions Pub Date : 2022-04-05 DOI:10.1080/17429145.2022.2056250

Yingbo Li, G. Guo, Hongwei Xu, Yingjie Zong, Shuwei Zhang, Linli Huang, R. Gao, R. Lu, Longhua Zhou, Chenghong Liu

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引用次数: 0

Abstract

ABSTRACT Barley (Hordeum vulgare L.) has been recognized as an ideal model plant to study the mechanism of environmental adaptation for crop improvement owing to its wide adaption to abiotic stresses. Abscisic acid is an essential hormone involved in many abiotic stresses. Here, an ABA-sensitive barley mutant (abas1) was generated by microspore embryogenesis. On exogenous ABA treatment, the seedlings of abas1 presented less plant height, root length, shoot and root dry matter than the wild type, and more genes showedigher expressional intensity and up-regulation models by RNA-Seq profiling. The gene ontology analysis revealed that the regulation of DEGs may lead to the activation of the ROS pathway and consumption of more energy in abas1, specifically. Furthermore, the up-regulation of two candidate genes (P450 and NsLTP) in abas1 may contribute to the variation of ABA sensitivity. Our findings provide more valuable material and information toward the understanding of the mechanism of ABA response in barley.

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大麦小孢子胚发生产生的aba敏感突变体的表型和转录组学特征

大麦（Hordeum vulgare L.）对非生物胁迫具有广泛的适应性，是研究作物改良环境适应机制的理想模式植物。脱落酸是一种与许多非生物胁迫有关的必需激素。本文通过小孢子胚胎发生产生了对ABA敏感的大麦突变体（abas1）。在外源ABA处理下，abas1的幼苗比野生型表现出更少的株高、根长、地上部和根干物质，并且更多的基因表现出更高的表达强度和RNA-Seq图谱的上调模型。基因本体论分析表明，DEGs的调节可能导致ROS途径的激活，并消耗更多的能量。此外，abas1中两个候选基因（P450和NsLTP）的上调可能有助于ABA敏感性的变化。我们的发现为理解大麦ABA反应机制提供了更有价值的材料和信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Plant Interactions PLANT SCIENCES-

CiteScore

5.30

自引率

6.20%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Plant Interactions aims to represent a common platform for those scientists interested in publishing and reading research articles in the field of plant interactions and will cover most plant interactions with the surrounding environment.