{"title":"Improving Uniformity of Cell Distribution in Post-Inkjet-Based Bioprinting","authors":"Jiachen Liu, Changxue Xu","doi":"10.1115/1.4063134","DOIUrl":null,"url":null,"abstract":"\n Advancements of additive manufacturing enable fabrication of in vitro biomimetic grafts leveraging biological materials and cells for various biomedical applications. The realization of such biofabrication typically requires time from minutes to hours depending on the scale and complexity of the models. During direct biofabrication, cell sedimentation with the resultant aggregation is extensively deemed to be one of the acute problems for precise and reliable inkjet-based bioprinting. It often results in highly unstable droplet formation, nozzle clogging, and non-uniformity of post-printing cell distribution. Our previous study has implemented active bioink circulation to mitigate cell sedimentation and aggregation within the bioink reservoir. This study focuses on the comparison of post-printing cell distribution within formed microspheres and one-layer sheets with and without active circulation. The experimental results have demonstrated the significant improvement in post-printing cell distribution under implemented active circulation. Moreover, the printed sheet samples are subject to 3-day incubation to investigate the effect of cell distribution on cell viability and proliferation. It shows that compared to non-uniform cell distribution the uniform cell distribution significantly improves cell viability (92% vs 77% at Day 3) and cell proliferation (3.3 times vs 1.7 times at Day 3). The preliminary results in this letter have demonstrated not only the high effectiveness of the active bioink circulation to improve post-printing cell distribution within microspheres and one-layer sheets, but also the critical role of the uniform post-printing cell distribution in promoting cell viability and proliferation.","PeriodicalId":16299,"journal":{"name":"Journal of Manufacturing Science and Engineering-transactions of The Asme","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Manufacturing Science and Engineering-transactions of The Asme","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1115/1.4063134","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, MANUFACTURING","Score":null,"Total":0}
引用次数: 0
Abstract
Advancements of additive manufacturing enable fabrication of in vitro biomimetic grafts leveraging biological materials and cells for various biomedical applications. The realization of such biofabrication typically requires time from minutes to hours depending on the scale and complexity of the models. During direct biofabrication, cell sedimentation with the resultant aggregation is extensively deemed to be one of the acute problems for precise and reliable inkjet-based bioprinting. It often results in highly unstable droplet formation, nozzle clogging, and non-uniformity of post-printing cell distribution. Our previous study has implemented active bioink circulation to mitigate cell sedimentation and aggregation within the bioink reservoir. This study focuses on the comparison of post-printing cell distribution within formed microspheres and one-layer sheets with and without active circulation. The experimental results have demonstrated the significant improvement in post-printing cell distribution under implemented active circulation. Moreover, the printed sheet samples are subject to 3-day incubation to investigate the effect of cell distribution on cell viability and proliferation. It shows that compared to non-uniform cell distribution the uniform cell distribution significantly improves cell viability (92% vs 77% at Day 3) and cell proliferation (3.3 times vs 1.7 times at Day 3). The preliminary results in this letter have demonstrated not only the high effectiveness of the active bioink circulation to improve post-printing cell distribution within microspheres and one-layer sheets, but also the critical role of the uniform post-printing cell distribution in promoting cell viability and proliferation.
期刊介绍:
Areas of interest including, but not limited to: Additive manufacturing; Advanced materials and processing; Assembly; Biomedical manufacturing; Bulk deformation processes (e.g., extrusion, forging, wire drawing, etc.); CAD/CAM/CAE; Computer-integrated manufacturing; Control and automation; Cyber-physical systems in manufacturing; Data science-enhanced manufacturing; Design for manufacturing; Electrical and electrochemical machining; Grinding and abrasive processes; Injection molding and other polymer fabrication processes; Inspection and quality control; Laser processes; Machine tool dynamics; Machining processes; Materials handling; Metrology; Micro- and nano-machining and processing; Modeling and simulation; Nontraditional manufacturing processes; Plant engineering and maintenance; Powder processing; Precision and ultra-precision machining; Process engineering; Process planning; Production systems optimization; Rapid prototyping and solid freeform fabrication; Robotics and flexible tooling; Sensing, monitoring, and diagnostics; Sheet and tube metal forming; Sustainable manufacturing; Tribology in manufacturing; Welding and joining