Effects of interferon inducible protein 16 (IFI16) on viral protein and pro-inflammatory cytokine expression in human T-cell leukemia virus type 1 (HTLV-1)-positive T cells

Q4 Immunology and Microbiology

中华微生物学和免疫学杂志 Pub Date : 2019-09-30 DOI:10.3760/CMA.J.ISSN.0254-5101.2019.09.005

Yuhan Cui, Yuhe Guan, Yue Liu, Ge Zhang, Fan Chen, Mengmeng Chen, Jing-Jing Sun, X. Ren, Bo Yang, Jie Wang

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引用次数: 0

Abstract

Objective To investigate the effects of interferon inducible protein 16 (IFI16), a cytosolic DNA sensor, on the expression of human T-cell leukemia virus type 1 (HTLV-1) proteins and pro-inflammatory cytokines in adult HTLV-1-positive T cells. Methods IFI16 expression in different HTLV-1-positive T cell lines was detected by immunoblot assay. Specific siRNA targeting the IFI16 gene was constructed and the gene silencing efficiency was detected by immunoblot assay. Expression of HTLV-1 Tax protein at mRNA and protein levels was respectively detected by real-time PCR and immunoblot assay after knocking down the expression of IFI16 in HTLV-1-positive T cells with siRNA. Expression of interferon (IFN)-α, IFN-γ, tumor necrosis factor (TNF)-α, Tax and Env were detected by real-time PCR. Results Compared with the HTLV-1-negative T cell line Jurkat, IFI16 expression was enhanced in the HTLV-1-positive T cell lines MT2, MT4 and C8166. Tax expression was increased, while that of IFN-α, IFN-γ and TNF-α was decreased in MT2 and MT4 cells after silencing the expression of IFI16 with siRNA. Conclusions IFI16 expression was increased in HTLV-1-positive MT2 and MT4 cells. Meanwhile, IFI16 promoted the production of interferon and pro-inflammatory cytokines and inhibited the expression of HTLV-1 proteins. Key words: Interferon inducible protein 16 (IFI16); Human T-cell leukemia lymphoma virus type 1 (HTLV-1); Interferon; T lymphocyte; TNF-α

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干扰素诱导蛋白16 (IFI16)对人T细胞白血病病毒1型(HTLV-1)阳性T细胞中病毒蛋白和促炎细胞因子表达的影响

目的探讨胞质DNA传感器干扰素诱导蛋白16 (IFI16)对成人HTLV-1阳性T细胞中人T细胞白血病病毒1型(HTLV-1)蛋白及促炎因子表达的影响。方法采用免疫印迹法检测IFI16在不同htlv -1阳性T细胞株中的表达。构建IFI16基因特异性siRNA，免疫印迹法检测基因沉默效果。用siRNA敲除HTLV-1阳性T细胞中IFI16的表达后，采用real-time PCR和免疫印迹法分别检测HTLV-1 Tax蛋白在mRNA和蛋白水平上的表达。实时荧光定量PCR检测干扰素(IFN)-α、IFN-γ、肿瘤坏死因子(TNF)-α、Tax、Env的表达。结果与htlv -1阴性T细胞株Jurkat相比，htlv -1阳性T细胞株MT2、MT4和C8166中IFI16的表达增强。用siRNA沉默IFI16表达后，MT2和MT4细胞中Tax表达增加，IFN-α、IFN-γ和TNF-α表达降低。结论htlv -1阳性MT2、MT4细胞中IFI16表达升高。同时，IFI16促进干扰素和促炎细胞因子的产生，抑制HTLV-1蛋白的表达。关键词:干扰素诱导蛋白16 (IFI16);人t细胞白血病淋巴瘤病毒1型(HTLV-1);干扰素;T淋巴细胞;肿瘤坏死因子-α

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来源期刊

中华微生物学和免疫学杂志 Immunology and Microbiology-Virology

CiteScore

0.50

自引率

0.00%

发文量

6906

期刊介绍： Chinese Journal of Microbiology and Immunology established in 1981. It is one of the series of journal sponsored by Chinese Medical Association. The aim of this journal is to spread and exchange the scientific achievements and practical experience in order to promote the development of medical microbiology and immunology. Its main contents comprise academic thesis, brief reports, reviews, summaries, news of meetings, book reviews and trends of home and abroad in this field. The distinguishing feature of the journal is to give the priority to the reports on the research of basic theory, and take account of the reports on clinical and practical skills.