Genotype-specific requirements for in vitro culture initiation and multiplication of Magnolia taxa

IF 0.9 Q4 ECOLOGY

Folia Oecologica Pub Date : 2020-04-01 DOI:10.2478/foecol-2020-0005

J. Konôpková, Dominika Košútová, P. Ferus

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引用次数: 2

Abstract

Abstract The influence of basal media composition, concentration of plant growth regulators (PGRs), and the developmental stage of primary explants (dormancy, stage of bud opening and fruit ripening) on the initiation phase of nine Magnolia genotypes, including M. stellata /Sieb. & Zucc./Maxim., M. × soulangeana ‘Rustica Rubra’, M. denudata Desr., M. × soulangeana ‘Alexandrina’, M. liliiflora Desr., M. officinalis var. biloba Rehd. & Wils., M. salicifolia Maxim., M. × soulangeana ‘Lennei’, and M. kobus DC, was evaluated. The highest efficiency of primary culture initiation of seven Magnolia genotypes (except for M. liliiflora and M. salicifolia) was achieved from primary explants collected in the bud opening stage. A high positive correlation was found between total tannins and efficiency of the primary culture initiation at the fruit ripening stage (r = 0.833). Standardi and Catalano medium (S2) with 0.5 mg l−1 of 6-benzylaminopurine (BAP) was the most appropriate for multiplication of M. × soulangeana ‘Alexandrina’, whereas tissue cultures of M. × soulangeana ‘Lennei’ proliferated and grew better on S2 medium with 1.0 mg l−1 of BAP and 1.0 g l−1 of polyvinylpyrrolidone. The requirements for the composition of basal media and concentration of PGRs in the initiation and multiplication stages of micropropagation of various Magnolia species and cultivars are genotype-specific.

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木兰属分类群体外培养起始和增殖的基因型特异性要求

摘要研究了基础培养基组成、植物生长调节剂（PGRs）浓度和初外植体发育阶段（休眠期、开芽期和果实成熟期）对9个木兰基因型（包括M.stellata/Sieb）启动期的影响Zucc/格言M.×soulangeana‘Rustica Rubra’，M.denudata Desr。，M.×soulangeana‘Alexandrina’，M.lililiflora Desr。，M.officinalis var.bloba Rehd.&威尔斯。，M.salicifolia Maxim。，M.×soulangeana‘Lennei’和M.kobus DC。除百合花和柳外，7个木兰基因型的初代培养起始效率最高。在果实成熟期，总单宁含量与原代培养启动效率呈正相关（r=0.833）。6-苄氨基嘌呤（BAP）0.5mg l−1的Standardi和Catalano培养基（S2）最适合亚历山大藻的增殖，×soulangeana‘Lennei’的组织培养物在含有1.0 mg l−1 BAP和1.0 g l−1聚乙烯吡咯烷酮的S2培养基上增殖和生长较好。在各种木兰属物种和品种的微繁殖起始和繁殖阶段，对基础培养基组成和PGRs浓度的要求是基因型特异性的。

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来源期刊

Folia Oecologica Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

2.50

自引率

15.40%

发文量

审稿时长

38 weeks

期刊介绍： Folia oecologica is a continuation of the journal Folia dendrologica published in the years 1974 - 1997. Folia oecologica is an international scientific journal. It has published original scientific works in the field of ecology oriented toward forest ecosystems, natural and urbanized environments, plants and animals.