Effects of combined 5-Fluorouracil and ZnO NPs on human breast cancer MCF-7 Cells: P53 gene expression, Bcl-2 signaling pathway, and invasion activity

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
S. Hoseinzadeh, E. Raeisi, Y. Lemoigne, E. Heidarian
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引用次数: 5

Abstract

Objective(s): The significant contribution of nanoparticles to cancer treatment has attracted therapeutic attention. The present study aimed to evaluate the synergistic effects of 5-fluorouracil (5-FU) and zinc oxide nanoparticles (ZnO NPs) as multimodal drug delivery on human breast cancer MCF-7 cells.Materials and Methods: In this in-vitro study, the impact of 5-FU and ZnO NPs in the single or combined forms was evaluated on cell viability, colony formation, apoptosis, p53 gene expression, and Bcl-2 signaling protein in MCF-7 breast cancer cell line using several techniques, such as MTT, clonogenic assay, flow cytometry, real-time quantitative polymerase chain reaction, and Western blot.Results: In this study, 5-FU combined with ZnO NPs showed synergistic effects against MCF-7 within 48 hours. In addition, the combination of 5-FU and ZnO NPs at the respective concentrations of 1 µM and 45 µg/ml exhibited significant apoptosis (79.53), p53 gene expression (3.6 folds), reduction of cell invasion (9.82), and plating efficiency (5), thereby leading to the significant reduction of cell viability (40±0.9) and decreased Bcl-2 anti-apoptotic protein relative to untreated control cells. Conclusion: According to the results, the synergistic effects of combined ZnO NPs and 5-FU on MCF-7 human breast cancer cells were exerted via Bcl-2 inhibition and the up-regulation of p53 expression.
5-氟尿嘧啶联合氧化锌NPs对人乳腺癌MCF-7细胞的影响:P53基因表达、Bcl-2信号通路和侵袭活性
目的:纳米颗粒对癌症治疗的重要贡献已引起治疗关注。本研究旨在评估5-氟尿嘧啶(5-FU)和氧化锌纳米颗粒(ZnO-NP)作为多模式药物递送对人类乳腺癌症MCF-7细胞的协同作用。材料与方法:本研究采用MTT、克隆形成试验、流式细胞术、实时定量聚合酶链反应和蛋白质印迹等技术,评价5-FU和ZnO NPs单体或联合形式对MCF-7乳腺癌症细胞系细胞活力、集落形成、凋亡、p53基因表达和Bcl-2信号蛋白的影响。结果:在本研究中,5-FU与ZnO NPs在48小时内对MCF-7表现出协同作用。此外,与未处理的对照细胞相比,分别浓度为1µM和45µg/ml的5-FU和ZnO NP的组合表现出显著的细胞凋亡(79.53)、p53基因表达(3.6倍)、细胞侵袭减少(9.82)和铺板效率(5),从而导致细胞存活力显著降低(40±0.9)和Bcl-2抗凋亡蛋白降低。结论:ZnO NPs和5-FU联合应用对MCF-7人乳腺癌症细胞的协同作用是通过抑制Bcl-2和上调p53表达来实现的。
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
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