N. P. Le, Huynh Anh Luu, Khanh Nguyen Huan Pham, Tan Loc Huynh, T. Chau, Thi Hong Mo Trinh, H. X. Nguyen, Trong Ngu Nguyen
{"title":"The effect of different media and temperature conditions for Salmonella bacteriophage preservation","authors":"N. P. Le, Huynh Anh Luu, Khanh Nguyen Huan Pham, Tan Loc Huynh, T. Chau, Thi Hong Mo Trinh, H. X. Nguyen, Trong Ngu Nguyen","doi":"10.12982/vis.2022.036","DOIUrl":null,"url":null,"abstract":"This research aimed to determine the optimal media and temperature conditions for the long-term storage of bacteriophages. In this study, the viability of Salmonella phages in 50% glycerol, 10% sodium chloride-magnesium sulfate (SM) buffer, and 5% dimethyl sulfoxide (DMSO) media at room temperature, 4oC, -20oC, and -80oC for 12 months was determined. In 50% glycerol, at the end of the experiment, no significant difference was found between four temperature conditions on phage density, ranging from 6.20-6.23 log10 PFU/mL (P>0.05). Under 10% SM medium, phage preservation at room temperature provided the optimum density at 6.31 log10 PFU/mL. In addition, phages preserved in a 5% DMSO medium were of similar density values across all temperature treatments. Still, their availability after 12 month-storage (88.0-88.5%) was significantly lower (P<0.05) than that of 50% glycerol and 10% SM. Moreover, for phage lysis capacity, low temperatures (4oC, -20oC, and -80oC) were superior to room temperature used for preservation. Considering the density, lysis capacity, and practical convenience, storing phages at 4°C in a 50% Glycerol medium is recommended.","PeriodicalId":36378,"journal":{"name":"Veterinary Integrative Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Integrative Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12982/vis.2022.036","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Veterinary","Score":null,"Total":0}
引用次数: 0
Abstract
This research aimed to determine the optimal media and temperature conditions for the long-term storage of bacteriophages. In this study, the viability of Salmonella phages in 50% glycerol, 10% sodium chloride-magnesium sulfate (SM) buffer, and 5% dimethyl sulfoxide (DMSO) media at room temperature, 4oC, -20oC, and -80oC for 12 months was determined. In 50% glycerol, at the end of the experiment, no significant difference was found between four temperature conditions on phage density, ranging from 6.20-6.23 log10 PFU/mL (P>0.05). Under 10% SM medium, phage preservation at room temperature provided the optimum density at 6.31 log10 PFU/mL. In addition, phages preserved in a 5% DMSO medium were of similar density values across all temperature treatments. Still, their availability after 12 month-storage (88.0-88.5%) was significantly lower (P<0.05) than that of 50% glycerol and 10% SM. Moreover, for phage lysis capacity, low temperatures (4oC, -20oC, and -80oC) were superior to room temperature used for preservation. Considering the density, lysis capacity, and practical convenience, storing phages at 4°C in a 50% Glycerol medium is recommended.