DNA barcoding of Philippine freshwater eels of families Anguillidae and Synbranchidae

Q3 Environmental Science

Philippine Journal of Systematic Biology Pub Date : 2022-05-17 DOI:10.26757/pjsb2021a15011

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引用次数: 1

Abstract

The study used morphological and genetic approach for the DNA barcoding analysis of the inter- and intra-species relationships among catadromous Philippine freshwater eels. Past DNA barcoding studies on Philippine anguillid eels came from Northern Luzon. This study aimed to determine the DNA barcodes of the freshwater eels of the Philippines using the cytochrome c oxidase 1 (CO1) gene of the mitochondrial DNA. Specimens were collected from six sites in the Philippines. Four Anguilla species – Anguilla bicolor pacifica Schmidt, 1928, A. celebesensis Kaup, 1856, A. interioris Whitney, 1938 and A. marmorata Quoy & Gaimard, 1824 and one Monopterus species– Monopterus javanensis Lacepéde, 1800 were collected and identified. Morphological features varied from the taxonomic guides for Anguilla celebesensis and A. interioris with their body color and fin length. Genetic divergence estimates using Kimura 2-parameter substitution model showed an intraspecific variation of 0–0.4% and interspecific variation of 4.1– 27.6%. The ML tree generated was similar to the previous studies and indicated the monophyly of the Indo–Pacific freshwater anguillid eel lineage. This study also reports the first genetic record of M. javanensis sampled from Batac, Ilocos Norte, Philippines.

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菲律宾淡水鳗Anguillidae和Synbranchidae科的DNA条形码

本研究采用形态学和遗传学方法对菲律宾淡水鳗鱼的种间和种内关系进行了DNA条形码分析。过去对菲律宾鳗的DNA条形码研究来自吕宋岛北部。本研究旨在利用线粒体DNA的细胞色素c氧化酶1（CO1）基因测定菲律宾淡水鳗鱼的DNA条形码。从菲律宾的六个地点采集了标本。收集并鉴定了四种安圭拉物种——双色和平安圭拉施密特，1928年，A.celesensis Kaup，1856年，A.interioris Whitney，1938年和A.marmorata Quoy&Gaimard，1824年，以及一种黄鳝物种——爪哇黄鳝Lacepéde，1800年。根据体色和鳍长的不同，安圭拉的形态特征各不相同。使用Kimura双参数替代模型进行的遗传差异估计显示，种内变异为0–0.4%，种间变异为4.1–27.6%。生成的ML树与先前的研究相似，表明印度-太平洋淡水鳗谱系为单系。本研究还报道了从菲律宾北伊洛科斯巴塔克采集的爪哇M.javanensis的第一个遗传记录。

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来源期刊

Philippine Journal of Systematic Biology Environmental Science-Ecology

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： The Philippine Journal of Systematic Biology (Print ISSN: 1908-6865; Online ISSN: 2508-0342) is an annual, peer-reviewed journal in English that publishes high quality reports of original research and reviews in the field of Taxonomy & Systematics, Ecology and Conservation Biology. The PJSB is a CHED recognized journal under CHED CMO 50 series of 2017. It is also included in the Clavariate Analysis (formerly a subsidiary of Thomson Reuters) Master Journal List under Zoological Record, BIOSIS Previews and Biological Abstracts.The PJSB is the official publication of the Association of Systematic Biologists of the Philippines (SEC Registration: Association of Philippine Taxonomists, Inc.). The organization financially supports the journal and all its endeavors. The journal aims to build up quality information on animal, plant and microbial diversity in the Philippines. Articles dealing with original research or reviews in Zoological, Botanical or Microbial Systematics, Ecology and Conservation Biology are welcome. Authors are advised to consult a recent issue of PJSB for the current format and style. All manuscripts are reviewed by at least two qualified referees.