CAPACIDAD BIOCONTROLADORA DE AISLAMIENTOS NATIVOS DE Trichoderma sp. CONTRA EL HONGO FITOPATÓGENO Alternaria alternata, AISLADO DE YERBA MATE (Ilex paraguariensis Saint Hil.)

IF 0.3 Q4 AGRONOMY
M. Castrillo, G. Bich, Gastón Sioli, P. Zapata, L. Villalba
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引用次数: 1

Abstract

Phytopathogenic fungi attack many agricultural crops such as yerba mate (Ilex paraguariensis Saint Hil.). An innovative alternative for pest control is the use of biocontrol fungi of Trichoderma genus. The objectives of the present work were to isolate the fungal agents that cause foliar lesions in yerba mate, and to evaluate the antagonistic capacity of native Trichoderma sp. isolates against pathogens in this crop. From diseased plant-tissues, a phytopathogenic fungus belonging to Alternaria alternata species was isolated and identified morphologically and molecularly. Pathogenicity assays were done on yerba mate plants in triplicate. Superficial mechanical lesions were made on them and the phytopathogenic isolation obtained (conidia and mycelium) was inoculated to evaluate their phytopathogenic capacity. New dual culture methods were determined to evaluate in vitro antagonism of Trichoderma. The in vitro biocontrol capacity of 30 Trichoderma isolates was evaluated against the phytopathogenic isolation obtained. As a positive control, a strain isolated from a commercial fungicide product based on T. harzianum was used. It was determined that 18 of the 30 isolates of Trichoderma inhibited the growth of the phytopathogen A. alternata by more than 50%, and presented statistically significant differences with respect to the positive control. These results confirm the effectiveness of using native biocontrol isolates adapted to the climatic and soil conditions where the phytopathogenic microorganisms are found.
木霉本地分离株对巴拉圭YERBA MATE (Ilex paraguariensis Saint Hil)植物病原真菌Alternaria alternata的生物防治能力。
植物病原真菌侵袭许多农作物,如巴拉圭马黛茶(巴拉圭冬青)。一种创新的害虫防治方法是利用木霉属的生物防治真菌。本研究的目的是分离引起马黛茶叶面损伤的真菌制剂,并评价本地木霉菌株对马黛茶病原菌的拮抗能力。从病害植物组织中分离出一种植物致病真菌,并进行了形态和分子鉴定。对三株马黛茶植株进行了致病性测定。对其进行表面机械损伤,接种获得的植物致病性分离物(分生孢子和菌丝体),评价其植物致病性。研究了新的双重培养方法,以评价木霉的体外拮抗作用。对30株木霉分离株的体外防菌能力进行了比较。阳性对照采用从商业杀菌剂产品中分离的一株哈氏芽孢杆菌。结果表明,30株木霉菌株中有18株对交替植物病原菌的抑制作用大于50%,与阳性对照差异有统计学意义。这些结果证实了使用适应发现植物病原微生物的气候和土壤条件的本地生物防治分离物的有效性。
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来源期刊
CiteScore
0.70
自引率
0.00%
发文量
23
期刊介绍: Revista Chile de Agricultura y Ciencias Veterinarias es una revista de acceso abierto (open access), que significa que su contenido está disponible en forma gratuita para los usuarios y sus instituciones. Los usuarios pueden leer, descargar, copiar, distribuir, imprimir, buscar, o establecer una conexión a los artículos sin necesidad de pedir autorización previa al editor o a los autores. Esto es de acuerdo con la definición de Budapest Open Access Initiative (BOAI). Los artículos se publican bajo una licencia de Creative Commons reconocimiento No Comercial 4.0 Internacional. Copyright: Se autoriza la reproducción y cita de los artículos publicados en Chilean Journal of Agricultural & Animal Sciences (ex Agro-Ciencia), siempre que se indique el nombre del autor(es), año, volumen, número y páginas. Las opiniones y afirmaciones expuestas en los trabajos representan exclusivamente los puntos de vista de los autores. La mención de productos o marcas comerciales en la revista no implica una recomendación por parte de la Universidad de Concepción.
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