CAPACIDAD BIOCONTROLADORA DE AISLAMIENTOS NATIVOS DE Trichoderma sp. CONTRA EL HONGO FITOPATÓGENO Alternaria alternata, AISLADO DE YERBA MATE (Ilex paraguariensis Saint Hil.)
M. Castrillo, G. Bich, Gastón Sioli, P. Zapata, L. Villalba
{"title":"CAPACIDAD BIOCONTROLADORA DE AISLAMIENTOS NATIVOS DE Trichoderma sp. CONTRA EL HONGO FITOPATÓGENO Alternaria alternata, AISLADO DE YERBA MATE (Ilex paraguariensis Saint Hil.)","authors":"M. Castrillo, G. Bich, Gastón Sioli, P. Zapata, L. Villalba","doi":"10.29393/chjaas37-26cbml50026","DOIUrl":null,"url":null,"abstract":"Phytopathogenic fungi attack many agricultural crops such as yerba mate (Ilex paraguariensis Saint Hil.). An innovative alternative for pest control is the use of biocontrol fungi of Trichoderma genus. The objectives of the present work were to isolate the fungal agents that cause foliar lesions in yerba mate, and to evaluate the antagonistic capacity of native Trichoderma sp. isolates against pathogens in this crop. From diseased plant-tissues, a phytopathogenic fungus belonging to Alternaria alternata species was isolated and identified morphologically and molecularly. Pathogenicity assays were done on yerba mate plants in triplicate. Superficial mechanical lesions were made on them and the phytopathogenic isolation obtained (conidia and mycelium) was inoculated to evaluate their phytopathogenic capacity. New dual culture methods were determined to evaluate in vitro antagonism of Trichoderma. The in vitro biocontrol capacity of 30 Trichoderma isolates was evaluated against the phytopathogenic isolation obtained. As a positive control, a strain isolated from a commercial fungicide product based on T. harzianum was used. It was determined that 18 of the 30 isolates of Trichoderma inhibited the growth of the phytopathogen A. alternata by more than 50%, and presented statistically significant differences with respect to the positive control. These results confirm the effectiveness of using native biocontrol isolates adapted to the climatic and soil conditions where the phytopathogenic microorganisms are found.","PeriodicalId":42485,"journal":{"name":"Chilean Journal of Agricultural & Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.3000,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chilean Journal of Agricultural & Animal Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29393/chjaas37-26cbml50026","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 1
Abstract
Phytopathogenic fungi attack many agricultural crops such as yerba mate (Ilex paraguariensis Saint Hil.). An innovative alternative for pest control is the use of biocontrol fungi of Trichoderma genus. The objectives of the present work were to isolate the fungal agents that cause foliar lesions in yerba mate, and to evaluate the antagonistic capacity of native Trichoderma sp. isolates against pathogens in this crop. From diseased plant-tissues, a phytopathogenic fungus belonging to Alternaria alternata species was isolated and identified morphologically and molecularly. Pathogenicity assays were done on yerba mate plants in triplicate. Superficial mechanical lesions were made on them and the phytopathogenic isolation obtained (conidia and mycelium) was inoculated to evaluate their phytopathogenic capacity. New dual culture methods were determined to evaluate in vitro antagonism of Trichoderma. The in vitro biocontrol capacity of 30 Trichoderma isolates was evaluated against the phytopathogenic isolation obtained. As a positive control, a strain isolated from a commercial fungicide product based on T. harzianum was used. It was determined that 18 of the 30 isolates of Trichoderma inhibited the growth of the phytopathogen A. alternata by more than 50%, and presented statistically significant differences with respect to the positive control. These results confirm the effectiveness of using native biocontrol isolates adapted to the climatic and soil conditions where the phytopathogenic microorganisms are found.
期刊介绍:
Revista Chile de Agricultura y Ciencias Veterinarias es una revista de acceso abierto (open access), que significa que su contenido está disponible en forma gratuita para los usuarios y sus instituciones. Los usuarios pueden leer, descargar, copiar, distribuir, imprimir, buscar, o establecer una conexión a los artículos sin necesidad de pedir autorización previa al editor o a los autores. Esto es de acuerdo con la definición de Budapest Open Access Initiative (BOAI). Los artículos se publican bajo una licencia de Creative Commons reconocimiento No Comercial 4.0 Internacional. Copyright: Se autoriza la reproducción y cita de los artículos publicados en Chilean Journal of Agricultural & Animal Sciences (ex Agro-Ciencia), siempre que se indique el nombre del autor(es), año, volumen, número y páginas. Las opiniones y afirmaciones expuestas en los trabajos representan exclusivamente los puntos de vista de los autores. La mención de productos o marcas comerciales en la revista no implica una recomendación por parte de la Universidad de Concepción.