IDENTIFICATION OF THE OIL PALM BAGWORM, Mahasena corbetti TAMS (LEPIDOPTERA: PSYCHIDAE) VIA MOLECULAR TECHNIQUES AND ITS BIOCONTROL ASSAY USING Bacillus thuringiensis

IF 1.3 4区农林科学 Q2 Agricultural and Biological Sciences

Journal of Oil Palm Research Pub Date : 2023-08-11 DOI:10.21894/jopr.2023.0036

Mohd Shawal THAKIB MAIDIN

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Abstract

The larvae of the bagworm Mahasena corbetti Tams (Lepidoptera: Psychidae) cause significant yield losses by defoliation of the oil palm crop in Malaysia. This necessitates the development of an effective and sustainable control strategies against this pest. The objective of this study was to identify M. corbetti via molecular techniques and evaluate the potential of a local strain of Bacillus thuringiensis (MPOB Bt1), as a biocontrol agent for the pest. Morphological and molecular analysis was performed to identify M. corbetti , using the mitochondrial cytochrome oxidase I (COI) gene for genetic identification. The results showed that M. corbetti isolate SH01 (GenBank accession number MN373269) had a high degree of similarity (98.52%, 0.0 E-value) with the COI gene of M. corbetti voucher GB270DN1795 (GenBank accession number MG574312) collected in India and 100% BOLD similarities with the COI gene of M. corbetti from Singapore (BOLD ID PSYCH113-12). Laboratory bioassay showed 100% mortality of M. corbetti larvae three days after treatment with MPOB Bt1 (10 8 spores/mL). The results demonstrate the potential of MPOB Bt1 as an environmentally friendly biopesticide against M. corbetti in oil palm, but optimisation and efficacy in other pests require further research. Furthermore, biocontrol can be used as part of an integrated pest management program against bagworms and other lepidopteran pests.

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油棕BAGWORM的分子鉴定及苏云金芽孢杆菌的防制试验

在马来西亚，袋虫的幼虫使油棕作物落叶，造成重大的产量损失。这就需要针对这种有害生物制定有效和可持续的控制战略。本研究的目的是通过分子技术鉴定M. corbetti，并评估当地菌株苏云金芽孢杆菌(MPOB Bt1)作为害虫生物防治剂的潜力。利用线粒体细胞色素氧化酶I (cytochrome oxidase I, COI)基因进行遗传鉴定。结果表明，分离株SH01 (GenBank登录号MN373269)与印度产M. corbetti凭证GB270DN1795 (GenBank登录号MG574312)的COI基因高度相似(98.52%，0.0 e值)，与新加坡产M. corbetti COI基因(BOLD ID PSYCH113-12)的BOLD相似度为100%。实验室生物测定显示，MPOB Bt1(10 8孢子/mL)处理3天后，corbetti M. corbetti幼虫死亡率为100%。结果表明，MPOB Bt1作为一种环境友好型生物农药对油棕中的corbetti有潜力，但对其他害虫的优化和功效有待进一步研究。此外，生物防治可作为防治袋虫和其他鳞翅目害虫的综合虫害管理计划的一部分。

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来源期刊

Journal of Oil Palm Research 农林科学-食品科技

CiteScore

2.60

自引率

30.80%

发文量

审稿时长

>12 weeks

期刊介绍： JOURNAL OF OIL PALM RESEARCH, an international refereed journal, carries full-length original research papers and scientific review papers on various aspects of oil palm and palm oil and other palms. It also publishes short communications, letters to editor and reviews of relevant books. JOURNAL OF OIL PALM RESEARCH is published four times per year, i.e. March, June, September and December.