Detection of Aedes aegypti Resistance towards Cypermethrin using Polymerase Chain Reaction (PCR) Techniques in Ambarawa Semarang Regency

Abstract

Ambarawa Sub-district is one of the endemic sub-districts since 2017 which always accounts for the highest DHF cases in Semarang Regency. The purpose of this study was to detect molecular resistance of Aedes aegypti towards cypermethrin using Polymerase Chain Reaction (PCR) technique. This study was conducted to look at the mutation in the gene VGSC Aedes aegypti. This research is a pure experimental research. The results showed that in Tambakboyo Village, 2 samples were susceptible (V / V), 7 samples were detected as homozygous (G/G) resistant, 1 sample was detected as heterozygous (V/G) resistant; in Kupang Village 5 samples were detected as being homozygous resistant (G/G) and 5 samples were detected as heterozygous resistant (V/G); and in Panjang Village 1 susceptible sample (V/V), 8 samples were detected homozygous resistant (G/G), 1 sample detected heterozygous resistant (V/G). Resistance is detected by mutations. Mutations have been found in the VGSC gene in codon V1016G. ©2020 Universitas Negeri Semarang pISSN 2252-6781 eISSN 2548-7604 Article Info Article History: Submitted October 2019 Accepted December 2019 Published January 2020