Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects

IF 3.1 3区 材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY
Jelena Filipović Tričković, M. Momčilović, G. Joksić, S. Živković, Bojana Ilić, M. Ognjanović, M. Novaković, Ana Valenta Šobot
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Abstract

Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.
激光烧蚀柠檬酸盐稳定银纳米粒子显示尺寸和浓度依赖的生物效应
银纳米颗粒(AgNPs)由于其抗菌和抗炎作用,特别是在牙科和伤口愈合方面,已被公认为具有广泛的生物学应用。许多特征决定了它们的有益或有毒潜力,例如它们的合成类型、大小、形态、涂层和浓度。大多数合成类型依赖于合成化学品的使用,这导致了它们的毒性。我们提出了一种以柠檬酸盐为稳定剂,通过脉冲激光液体烧蚀(PLAL)从银靶“绿色”合成AgNPs的环保方法。由于AgNPs已经在牙科领域有许多应用,我们研究了它们对牙龈上生物膜形成细菌和已知引起耐药性牙齿感染的菌株的抗菌作用。评估了它们对人类成纤维细胞的细胞毒性、增殖(通过XTT和Ki-67免疫荧光测定)、促/抗氧化平衡和脂质过氧化(通过PAB和LPP测定)的影响。AgNPs1(21 nm)和AgNPs2(15 nm)的球形纳米颗粒。测试的最高剂量的较小纳米颗粒(AgNPs2)不仅对测试的口腔细菌菌株显示出有效的抗菌作用,而且对成纤维细胞也显示出促氧化和细胞毒性作用。较低的剂量不会影响细菌的存活,但会增加细胞增殖和代谢活性,并显示出抗氧化作用,这表明不同浓度显示出基本相反的效果。与较大的AgNPs1相比,较小的AgNPs2具有更强大的生物效应,这表明尺寸在其活性中起着关键作用。这种相反的结果可能对其医学应用很重要,高浓度可用于抑制牙齿生物膜的形成、抵抗性牙齿感染以及增殖条件,而低剂量可有益于治疗萎缩性和炎症性疾病。最后,我们证明了银靶向的PLAL,使用柠檬酸盐作为稳定剂,可以产生具有生物活性的纳米颗粒,根据其大小和浓度,这些纳米颗粒可以有许多应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nanomaterials and Nanotechnology
Nanomaterials and Nanotechnology NANOSCIENCE & NANOTECHNOLOGY-MATERIALS SCIENCE, MULTIDISCIPLINARY
CiteScore
7.20
自引率
21.60%
发文量
13
审稿时长
15 weeks
期刊介绍: Nanomaterials and Nanotechnology is a JCR ranked, peer-reviewed open access journal addressed to a cross-disciplinary readership including scientists, researchers and professionals in both academia and industry with an interest in nanoscience and nanotechnology. The scope comprises (but is not limited to) the fundamental aspects and applications of nanoscience and nanotechnology
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