C. Úsuga-Monroy, J. J. Echeverri, A. López-Herrera
{"title":"El componente racial influencia la resistencia a la infección con el virus de la leucosis bovina","authors":"C. Úsuga-Monroy, J. J. Echeverri, A. López-Herrera","doi":"10.15446/RFMVZ.V65N2.75632","DOIUrl":null,"url":null,"abstract":"espanolEl virus de la leucosis bovina (VLB) es un retrovirus que afecta principalmente el ganado lechero, reduciendo la produccion de leche entre el 2,5 y 5%. La raza criolla colombiana Blanco Orejinegro (BON) es una raza rustica, bien adaptada, que ha mostrado resistencia in vitro a las infecciones ocasionadas por los virus de la fiebre aftosa y la estomatitis vesicular, asi como las originadas por la bacteria Brucella abortus. El objetivo del presente estudio fue determinar si la raza BON y su cruce con Holstein son resistentes a la infeccion por el VLB. Se tomaron 124 muestras de sangre (59 Holstein, 40 BON y 25 BON x HOL) del mismo hato, se extrajo el DNA y se realizo una PCR-anidada correspondiente a una region del gen env de VLB. Se obtuvo un fragmento de 444 pb en los animales positivos. La prevalencia molecular del hato fue 33% para VLB. Se encontro diferencia significativa para infeccion por VLB entre los tres grupos raciales (p EnglishThe bovine leukemia virus (BLV) is a retrovirus that primarily affects dairy cattle, reducing milk production between 2.5 and 5%. The Colombian Blanco Orejinegro (BON) is a well-adapted, rustic, creole breed resistant to in vitro infections of Foot-and-mouth disease virus and vesicular stomatitis virus, as well as to Brucella abortus. This study aimed to determine if the crossing of BON and Holstein breeds is resistant to infection by BLV. Blood samples of 124 individuals (59 Holstein, 40 BON, and 25 BON x HOL) of the same herd were taken. The DNA was extracted, and a nested PCR was performed related to a region of the env gene of BLV. A fragment of 444 bp was obtained for positives animals. The molecular in-herd prevalence was 33% for BLV. A significant difference for BLV infection was found among the groups (p","PeriodicalId":30232,"journal":{"name":"Revista de la Facultad de Medicina Veterinaria y de Zootecnia","volume":"65 1","pages":"130-139"},"PeriodicalIF":0.0000,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista de la Facultad de Medicina Veterinaria y de Zootecnia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15446/RFMVZ.V65N2.75632","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
espanolEl virus de la leucosis bovina (VLB) es un retrovirus que afecta principalmente el ganado lechero, reduciendo la produccion de leche entre el 2,5 y 5%. La raza criolla colombiana Blanco Orejinegro (BON) es una raza rustica, bien adaptada, que ha mostrado resistencia in vitro a las infecciones ocasionadas por los virus de la fiebre aftosa y la estomatitis vesicular, asi como las originadas por la bacteria Brucella abortus. El objetivo del presente estudio fue determinar si la raza BON y su cruce con Holstein son resistentes a la infeccion por el VLB. Se tomaron 124 muestras de sangre (59 Holstein, 40 BON y 25 BON x HOL) del mismo hato, se extrajo el DNA y se realizo una PCR-anidada correspondiente a una region del gen env de VLB. Se obtuvo un fragmento de 444 pb en los animales positivos. La prevalencia molecular del hato fue 33% para VLB. Se encontro diferencia significativa para infeccion por VLB entre los tres grupos raciales (p EnglishThe bovine leukemia virus (BLV) is a retrovirus that primarily affects dairy cattle, reducing milk production between 2.5 and 5%. The Colombian Blanco Orejinegro (BON) is a well-adapted, rustic, creole breed resistant to in vitro infections of Foot-and-mouth disease virus and vesicular stomatitis virus, as well as to Brucella abortus. This study aimed to determine if the crossing of BON and Holstein breeds is resistant to infection by BLV. Blood samples of 124 individuals (59 Holstein, 40 BON, and 25 BON x HOL) of the same herd were taken. The DNA was extracted, and a nested PCR was performed related to a region of the env gene of BLV. A fragment of 444 bp was obtained for positives animals. The molecular in-herd prevalence was 33% for BLV. A significant difference for BLV infection was found among the groups (p