Chemical Composition and Antiproliferative Activity of Rhizome and Frond Essential Oils of Anemia schimperiana subsp. wightiana, a Rare Fern Endemic to South India

IF 0.9 Q4 CHEMISTRY, MEDICINAL

Journal of Biologically Active Products from Nature Pub Date : 2023-03-04 DOI:10.1080/22311866.2023.2211046

S. A. Nair, Rajani Kurup Sukumaryamma Remadevi, Smrithi S. Lal, R. Raju, R. Antony, Sabulal Baby

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引用次数: 1

Abstract

Abstract Anemia schimperiana C.Presl subsp. wightiana (Gardener) Fraser-Jenkins is a rhizomatous fern endemic to south India. Its rhizome and frond (leaf) essential oils (EOs) were isolated and characterized by gas chromatographic techniques. Thirty compounds (99.1%) of the rhizome EO were characterized, with myrtenyl acetate (50.6%), α-pinene (26.0%), myrtenol (7.3%) and trans-pinocarveol (5.6%) as its major constituents. Twenty-four compounds (97.8%), with trans-pinocarveol (43.0%), pinocarvone (17.3%) and myrtenyl acetate (15.8%), were identified in the frond EO. In MTT assay, rhizome EO showed prominent cytotoxicity against C6 (CD50 86.0 µg/mL) and DLA (CD50 6.1 µg/mL) cells. Fluorescent microscopic and caspase-3 activity assays demonstrated apoptotic cell death induced by the rhizome EO in DLA cells. MTT assay of myrtenyl acetate, α-pinene and myrtenol against DLA cells displayed CD50s of 7.4, 0.9 and 20.5 µg/mL, respectively, revealing α-pinene as the prominent constituent causing antiproliferative activity of the rhizome EO. This is the first report on the chemistry and antiproliferative activity of this rare aromatic fern endemic to south India. GRAPHICAL ABSTRACT

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刺花属贫血植物根、叶精油的化学成分及抗增殖活性研究。南印度特有的一种稀有蕨类植物

文章摘要：川银莲花亚种。Fraser-Jenkins是印度南部特有的一种根茎蕨类植物。采用气相色谱技术对其根茎和叶（叶）精油进行了分离和表征。以乙酸月桂烯酯（50.6%）、α-蒎烯（26.0%）、月桂醇（7.3%）和反式皮诺卡韦醇（5.6%）为主要成分，对根状茎EO的30个化合物（99.1%）进行了表征。在叶片EO中鉴定出24种化合物（97.8%），包括反式皮诺卡韦醇（43.0%）、皮诺香芹酮（17.3%）和乙酸月桂烯酯（15.8%）。在MTT分析中，根状茎EO对C6（CD50 86.0µg/mL）和DLA（CD50 6.1µg/mL）细胞显示出显著的细胞毒性。荧光显微镜和胱天蛋白酶-3活性测定显示DLA细胞中的根状茎EO诱导细胞凋亡。乙酸月桂烯酯、α-蒎烯和月桂醇对DLA细胞的MTT分析显示，CD50分别为7.4、0.9和20.5µg/mL，表明α-松果烯是引起根状茎EO抗增殖活性的主要成分。这是首次报道这种罕见的印度南部特有芳香蕨类植物的化学成分和抗增殖活性。图形摘要

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来源期刊

Journal of Biologically Active Products from Nature Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

2.10

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0.00%

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