Molecular detection, isolation, and pathology of bovine tuberculosis in an organized farm in Assam, India

IF 0.1 0 VETERINARY SCIENCES
N. A. Kader, Samir Das, A. G. Barua, B. Dutta, R. Hazarika, N. Barman, S. N. Abedin, Syed A. Arif, Pranjal M. Nath, U. Rajkhowa
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引用次数: 0

Abstract

: Bovine tuberculosis (bTB) is a well-known zoonotic disease that affects cattle all over the world and results in significant economic loss, particularly in impoverished nations. The present communication describes the pathology, isolation and molecular detection of Mycobacterium in an organized farm of Assam which has the previous records on animals with confirmed M. bovis infection. During the period 2020-2021, a total of 40 animals (4 males and 36 females) of one year and above were included in the present study for screening of bovine tuberculosis by single intradermal comparative tuberculin test (SICCT). The milk, nasal swabs were collected from only tuberculin positive cattle and the tissue samples from necropsied animal and then processed for bacteriology, histopathology and molecular detection from direct samples. Out of 40, four cows showed positive reactor by SICCT and out of these four, one animal died. At necropsy, there was presence of circumscribed yellowish white lesions of various sizes and numbers. The smear prepared from granulomatous tissue samples showed the presence of acid-fast bacilli by Ziehl–Neelsen stain. Mycobacterium could be isolated from tissue samples. The DNA extracted from the samples could amplify Mycobacteria genus specific hsp65 gene and MTBC specific a 123-bp segment of the
印度阿萨姆邦一个有组织农场牛结核病的分子检测、分离和病理学
:牛结核病(bTB)是一种众所周知的人畜共患疾病,影响着全世界的牛,并造成重大经济损失,尤其是在贫困国家。本通讯描述了阿萨姆邦一个有组织的农场中分枝杆菌的病理学、分离和分子检测,该农场以前有确诊牛分枝杆菌感染动物的记录。在2020-2021年期间,共有40只一岁及以上的动物(4只雄性和36只雌性)被纳入本研究,通过单皮内比较结核菌素试验(SICCT)筛查牛结核病。仅从结核菌素阳性牛身上采集牛奶、鼻拭子,从尸检动物身上采集组织样本,然后从直接样本中进行细菌学、组织病理学和分子检测。在40头奶牛中,有4头奶牛的SICCT反应呈阳性,其中1头死亡。尸检时,存在各种大小和数量的局限性黄白色病变。根据肉芽肿组织样本制备的涂片,Ziehl–Neelsen染色显示存在抗酸杆菌。分枝杆菌可以从组织样本中分离出来。从样品中提取的DNA可以扩增分枝杆菌属特异性hsp65基因和MTBC特异性的123bp片段
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
16
审稿时长
20 weeks
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