Characterization of Venom Components of Two Medically Important Scorpion Species, Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897)

IF 0.2 4区农林科学 Q4 ENTOMOLOGY

Journal of the Kansas Entomological Society Pub Date : 2021-05-24 DOI:10.2317/0022-8567-93.3.230

H. M. Tahir, Saadia Tobassum, Shaukat Ali, Muhammad Tariq Zahid, M. Arshad, Qurratulann Afza Gardner, M. Ahsan

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引用次数: 1

Abstract

ABSTRACT: Scorpion venom is being used for many biomedical applications. In the present study, venom components of Hottentota tamulus (Fabricius, 1798) and Androctonus finitimus (Pocock, 1897) were characterized. Venom was extracted using the electrical stimulation method and subjected to High Performance Liquid Chromatography (HPLC) for characterization of its components from which dominant fractions were collected and dried in a concentrator. Crude venom and selected fractions were further analyzed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to obtain mass fingerprint data. Over 100 components ranging from 3,000 – 8,000 m/z in molecular masses were detected in the venom of both scorpion species. Some of them were analogous to a short chain and long chain toxins, which act on potassium and sodium ion channels, respectively. The venom components may be used for the development of novel drugs, e.g. drugs for neurological disorders, cardiovascular diseases, analgesics and cancer.

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两种医学上重要的蝎子的毒液成分特征，Hottentota tamulus（Fabricius，1798）和Androctonus finitimus（Pocock，1897）

摘要：蝎毒被广泛应用于生物医学领域。在本研究中，对霍滕托塔（Fabricius，1798）和安德克通（Androctonus finitimus，Pocock，1897）的毒液成分进行了表征。使用电刺激法提取毒液，并进行高效液相色谱（HPLC）以表征其成分，从中收集主要组分并在浓缩器中干燥。通过基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOF MS）对粗毒液和所选组分进行进一步分析，以获得质量指纹数据。在这两种蝎子的毒液中都检测到了100多种分子量在3000–8000 m/z之间的成分。其中一些类似于短链和长链毒素，分别作用于钾离子通道和钠离子通道。毒素组分可用于开发新的药物，例如用于神经疾病、心血管疾病、止痛药和癌症的药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Kansas Entomological Society 生物-昆虫学

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of the Kansas Entomological Society is a publication of the Kansas (Central States) Entomological Society, publishes research on all aspects of the sciences of entomology, and has world-wide authorship and readership.