Isolation Rate of Campylobacter Spp. and Detection of Virulence Genes of Campylobacter jejuni Across the Broiler Chain

IF 0.4 Q4 VETERINARY SCIENCES

Macedonian Veterinary Review Pub Date : 2021-07-17 DOI:10.2478/macvetrev-2021-0020

L. Angelovski, Zagorka Popova, K. Blagoevska, S. Mojsova, Marija Ratkova Manovska, M. Prodanov, D. Jankuloski, P. Sekulovski

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引用次数: 1

Abstract

Abstract The aim of the study was to identify the isolation rate of thermotolerant campylobacters in a small-scale broiler-meat production farm over a one-year period. The second deliverable of the study was to determine the potential virulence markers. The laboratory investigation was performed on 283 samples (cloacal swabs, caeca, carcass swabs) collected on three sampling points (farm, slaughter line, and cold storage). The isolates obtained with the conventional microbiological method were confirmed with multiplex PCR for identification of campylobacters. The presence of 10 virulence genes was analyzed in the C. jejuni isolates (flaA, racR, virB11, dnaJ, wlaN, cadF, ciaB, cdtA, cdtB, cdtC). Out of 283 samples, 169 (59.7%) were confirmed as Campylobacter spp., 111 (39.2%) C. jejuni, and 43 (15.2%) C. coli. C. jejuni was the most prevalent in all sampling points. Campylobacter spp. showed a characteristically seasonal prevalence with the highest isolation rate during the warmer period of the year. We detected the cadF and ciaB genes in all C. jejuni isolates. The flaA gene was present in 50% of the examined strains. The cdt genes (cdtA, cdtB, and cdtC) were confirmed in 52.8%, 52.8%, and 47.2% of the C. jejuni strains, respectively. C. jejuni showed 15 profiles of virulence patterns with four predominant profiles.

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肉鸡全链空肠弯曲杆菌分离率及毒力基因检测

摘要本研究的目的是确定一个小型肉鸡养殖场一年内耐热弯曲杆菌的分离率。该研究的第二个成果是确定潜在的毒力标记。对在三个采样点（农场、屠宰线和冷藏库）采集的283份样本（泄殖腔拭子、盲肠、胴体拭子）进行了实验室调查。用常规微生物学方法获得的分离株用多重聚合酶链式反应鉴定弯曲杆菌。在空肠弯曲菌分离株中分析了10个毒力基因的存在（flaA、racR、virB11、dnaJ、wlaN、cadF、ciaB、cdtA、cdtB、cdtC）。在283个样本中，169个（59.7%）被确认为弯曲杆菌属，111个（39.2%）为空肠弯曲菌，43个（15.2%）为大肠杆菌。空肠弯曲菌在所有采样点中最为普遍。弯曲杆菌属表现出典型的季节性流行，在一年中较温暖的时期分离率最高。我们在所有空肠弯曲菌分离株中检测到了cadF和ciaB基因。flaA基因存在于50%的检测菌株中。cdt基因（cdtA、cdtB和cdtC）分别在52.8%、52.8%和47.2%的空肠弯曲菌菌株中得到证实。空肠弯曲菌表现出15种毒力模式，其中4种占优势。

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