Production of biosurfactant by Bacillus megaterium and its correlation with lipid peroxidation of Lactuca sativa

Q1 Earth and Planetary Sciences

Egyptian Journal of Petroleum Pub Date : 2022-06-01 DOI:10.1016/j.ejpe.2022.03.001

Huda S. El-Sheshtawy , Hesham M. Mahdy , Ahmed R. Sofy , Mahmoud R. Sofy

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引用次数: 18

Abstract

The biosurfactant-producing bacterial isolate was isolated from an oil-contaminated water sample. This study aimed to determine the production of biosurfactant and its effect on the plant growth via the biosorption of some heavy metals. The isolate was identified by 16S rRNA gene sequence analysis as Bacillus megaterium ATTC 14581. The surface activity of the produced biosurfactant was determined. The biosurfactant was recovered from the cell-free culture of a bacterial strain at 1.5 g/L. The extracted biosurfactant shows potential stability towards some factors, like temperature and salinity. The FTIR analysis confirmed the production of surfactin biosurfactant. The impact of B. megaterium biosurfactant on Pb and Ar significantly increases morphological features, proline, and antioxidant enzymes, while a significant decrease in lipid peroxidation, H₂O₂, and O₂.

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巨型芽孢杆菌生产生物表面活性剂及其与油菜脂质过氧化的关系

从受石油污染的水样中分离得到一株生物表面活性剂分离菌。本研究旨在探讨生物表面活性剂对重金属的生物吸附作用及其对植物生长的影响。经16S rRNA基因序列分析，该分离物为巨型芽孢杆菌ATTC 14581。测定了所得生物表面活性剂的表面活性。从菌株的无细胞培养中以1.5 g/L的浓度回收生物表面活性剂。所提取的生物表面活性剂对温度、盐度等因素具有潜在的稳定性。FTIR分析证实生产了表面活性剂surfactin。巨芽孢杆菌生物表面活性剂对Pb和Ar的影响显著增加了形态特征、脯氨酸和抗氧化酶，同时显著降低了脂质过氧化、H2O2和O2。

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来源期刊

Egyptian Journal of Petroleum Earth and Planetary Sciences-Geochemistry and Petrology

CiteScore

7.70

自引率

0.00%

发文量

审稿时长

84 days

期刊介绍： Egyptian Journal of Petroleum is addressed to the fields of crude oil, natural gas, energy and related subjects. Its objective is to serve as a forum for research and development covering the following areas: • Sedimentation and petroleum exploration. • Production. • Analysis and testing. • Chemistry and technology of petroleum and natural gas. • Refining and processing. • Catalysis. • Applications and petrochemicals. It also publishes original research papers and reviews in areas relating to synthetic fuels and lubricants - pollution - corrosion - alternate sources of energy - gasification, liquefaction and geology of coal - tar sands and oil shale - biomass as a source of renewable energy. To meet with these requirements the Egyptian Journal of Petroleum welcomes manuscripts and review papers reporting on the state-of-the-art in the aforementioned topics. The Egyptian Journal of Petroleum is also willing to publish the proceedings of petroleum and energy related conferences in a single volume form.