Expression of FNDC5/Irisin in pancreatic cancer and its relationship with clinicopathological features

Q4 Medicine

中华肝胆外科杂志 Pub Date : 2019-12-28 DOI:10.3760/CMA.J.ISSN.1007-8118.2019.12.009

Huaiteng Xiao, Deguo Zhang, Shunchang Zhou, Jingxiong Zhao, Guangjun Shi

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引用次数: 0

Abstract

Objective To investigate the expression and distribution of FNDC5/Irisin in pancreatic cancer tissues and adjacent tissues, and to analyze its correlation with clinicopathological features. Methods Collection of archived wax blocks from 64 patients diagnosed with pancreatic cancer after surgical treatment from January 2015 to December 2018 in the Department of Pathology, Affiliated Qingdao Municipal Hospital of Qingdao University, and 30 tissues collected intraoperatively from January 2016 to December 2018 Samples, all collected samples included tumor tissue and corresponding adjacent tissues (>2 cm from the tumor edge). Real-time quantitative PCR (qRT-PCR) and immunohistochemistry were used to detect the expression of FNDC5/Irisin mRNA and its positivity in pancreatic cancer tissues and adjacent tissues, and to analyze its relationship with clinicopathological features of pancreatic cancer. Results qRT-PCR showed that the expression of FNDC5/Irisin mRNA in pancreatic cancer tissues was higher than that in the corresponding adjacent pancreatic tissues, the difference was statistically significant (P 0.05), but FNDC5/Irisin expression was associated with liver and lymph node metastasis (P<0.05). Conclusion The positivity of FNDC5/Irisin in pancreatic cancer tissues is significantly higher than that in adjacent pancreatic tissues, and it is correlated with liver and lymph node metastasis in pancreatic cancer. Key words: Pancreatic neoplasms; FNDC5/Irisin; Immunohistochemistry

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FNDC5/Irisin在胰腺癌中的表达及其与临床病理特征的关系

目的探讨FNDC5/Irisin在癌症组织及癌旁组织中的表达和分布，并分析其与临床病理特征的相关性。方法收集青岛大学附属青岛市立医院病理科2015年1月至2018年12月收治的64例癌症胰腺癌患者术后蜡块存档，以及2016年1月～2018年12月份术中收集的30例组织标本，所有采集的样本包括肿瘤组织和相应的邻近组织（距离肿瘤边缘>2cm）。应用实时定量PCR（qRT-PCR）和免疫组织化学方法检测FNDC5/Irisin mRNA在胰腺癌癌症组织及癌旁组织中的表达及其阳性表达，并分析其与癌症临床病理特征的关系。结果qRT-PCR显示胰腺癌症组织中FNDC5/Irisin mRNA的表达高于相应癌旁组织，差异有统计学意义（P＜0.05），结论胰腺癌癌症组织中FNDC5/Irisin阳性率明显高于癌旁组织，且与癌症的肝、淋巴结转移有关。关键词：胰腺肿瘤；FNDC5/Irisin；免疫组织化学

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来源期刊

中华肝胆外科杂志 Medicine-Gastroenterology

CiteScore

0.20

自引率

0.00%

发文量

7101

期刊介绍： Chinese Journal of Hepatobiliary Surgery is an academic journal organized by the Chinese Medical Association and supervised by the China Association for Science and Technology, founded in 1995. The journal has the following columns: review, hot spotlight, academic thinking, thesis, experimental research, short thesis, case report, synthesis, etc. The journal has been recognized by Beida Journal (Chinese Journal of Humanities and Social Sciences). Chinese Journal of Hepatobiliary Surgery has been included in famous databases such as Peking University Journal (Chinese Journal of Humanities and Social Sciences), CSCD Source Journals of China Science Citation Database (with Extended Version) and so on, and it is one of the national key academic journals under the supervision of China Association for Science and Technology.