In vivo healing potential of Vitis Vinifera L. and Punica Granatum L. fruit extracts in excision and burn models in rabbits

Abstract

Abstract Aim. The present study was carried out to evaluate the wound healing potential of crude Hydroalcoholic fruit extracts of Grape (Vitis vinifera L.) and Pomegranate (Punica granatum L.) in experimental animals. Material and methods. Extracts were formulated as ointments (250 mg), and their wound healing properties were determined by using two models: i.e. excision and burn models, in rabbits. Wound healing potential was assessed by measuring rate of wound contraction (percentage wound contraction) and the epithelialization period. Tukey test (HSD) test was used to analyze the obtained results. The value of p <0.05 was considered as a level of significance. The reference standard was wadded Cicatryl® ointment. Results. The result showed that crude fruit extracts of Vitis vinifera L. and Punica granatum L. exhibited a significant (p <0.0001) rate of wound contraction and significant (p<0.0001) decrease in the period of epithelialization compared to the control (untreated) group in both the models studied. Conclusion. Our results suggest that topical treatment of fruit extracts of Vitis vinifera L. and Punica granatum L. accelerate wound healing activity, thus our study supports their traditional use. Both extracts can be used as a potential source of wound healing therapeutics.