PmSOC1s and PmDAMs participate in flower bud dormancy of Prunus mume by forming protein complexes and responding to ABA
IF 0.7
4区 农林科学
Q4 HORTICULTURE
引用次数: 2
Abstract
and dormancy processes, such as SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and Dormancy Associated MADS-box (DAM) genes. Here, the expression patterns of PmSOC1s and PmDAMs er bud dormancy were analyzed. They had a similar expression trend, which gradually decreased in the process and positively correlated with the content of ABA. Besides, several ABA-responsiveness elements were predicted in promoter sequences of PmSOC1s and PmDAMs. The expression level of PmSOC1s and PmDAMs under ABA treatment was detected by qRT-PCR. Results showed that PmSOC1-2, PmSOC1-3 and PmDAM6 were highly sensitive to ABA. Moreover, PmSOC1-1, PmSOC1-2, PmSOC1-3 were detected in the nucleus of the cells in the Nicotiana benthamiana leaves through transient transformation. Furthermore, the results of yeast two-hybrid showed that there was a strong interaction between PmSOC1-2 and PmDAM6. Our results indicated thatPmSOC1s和PmDAMs通过形成蛋白质复合物和对ABA的反应参与梅花的花蕾休眠
和休眠过程，如CONSTANS 1过表达抑制因子(SOC1)和休眠相关MADS-box (DAM)基因。本文分析了pmsoc1和PmDAMs在芽体休眠中的表达规律。它们的表达趋势相似，在此过程中逐渐降低，且与ABA含量呈正相关。此外，在pmsoc1和PmDAMs的启动子序列中预测了几个aba响应元件。采用qRT-PCR检测ABA处理下pmsoc1和PmDAMs的表达水平。结果表明，PmSOC1-2、PmSOC1-3和PmDAM6对ABA高度敏感。PmSOC1-1、PmSOC1-2、PmSOC1-3通过瞬时转化在烟叶细胞核中检测到。酵母双杂交结果表明，PmSOC1-2与PmDAM6之间存在较强的相互作用。我们的研究结果表明
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来源期刊
CiteScore
1.90
自引率
11.10%
发文量
48
审稿时长
>36 weeks
期刊介绍：
eJHS publishes original research articles and reviews on significant plant science discoveries and new or modified methodologies and technologies with a broad international and cross-disciplinary interest in the scope of global horticulture. eJHS focus is on applied and fundamental aspects of the entire food value chain, ranging from breeding, production, processing, trading to retailing of horticultural crops and commodities in temperate and Mediterranean regions. In particular eJHS emphasizes integrated crop management strategies during the preharvest and postharvest stages, aiming at sustainably increasing the quantity and quality of horticultural products. The eJHS publishes peer-reviewed articles, reviews and special thematic issues in the following science areas:
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cropping systems, including crop management practices;
plant/fruit growth and development, including interactions with environments and organisms;
plant protection and plant nutrition;
irrigation and plant water relation;
whole plant physiology and source/sink relation;
urban horticulture/protected cultivation;
fruit ripening and product quality;
storage technologies, including postharvest disorders;
food processing technologies/horticultural engineering;
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