Virulence of Soybean Aphid, Aphis glycines (Hemiptera: Aphididae) Clones on Detached Leaves and Whole Plants

IF 0.2 4区 农林科学 Q4 ENTOMOLOGY
D. Lagos-Kutz, M. Pawlowski, B. Diers, Swapna R. Purandare, K. Tilmon, G. Hartman
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引用次数: 9

Abstract

ABSTRACT: Since the discovery of the invasive soybean aphid, Aphis glycines Matsumura, in the USA in 2000, populations have expanded throughout the Midwestern USA and Canada. Its negative economic impact on soybean [Glycine max (L.) Merr.] yield provides the impetus to continue investigating the effectiveness of host plant resistance which has been affected by soybean aphid biotypes that overcome soybean plants containing Rag (resistance to A. glycines) genes. In this study, 14 aphid clones collected on soybean and buckthorn (Rhamnus cathartica L.) plants along with four known aphid biotypes (from our stock collection) were evaluated in no-choice assays by quantifying the number of aphids on soybean genotypes with Rag genes and the susceptible cultivar Williams 82. No-choice assays were conducted using detached leaves and whole plants. Inconsistent responses of the biotypes from the stock collection by using detached leaves confirm the efficacy of whole plant assays to accurately differentiate biotypes. None of the biotypes or field clones from Illinois, Indiana and South Dakota overcame the resistance of soybean genotypes LD14-8004 (Rag1) and PI437696. A soybean aphid clone from Wooster, Ohio readily colonized LD14-8002 (Rag2), but did not overcome any of the other resistance genes indicating that it is different from biotypes 3 and 4, which also overcome LD14-8002 (Rag2) and, respectively, LD14-8004 (Rag1) and LD14-8006 (Rag3).
大豆蚜、大豆蚜(半翅目:蚜科)无性系对离体叶片和全株的毒力
摘要:自2000年在美国发现入侵性大豆蚜松村蚜以来,种群数量已扩展到美国中西部和加拿大。它对大豆[Glycine max(L.)Merr.]产量的负面经济影响为继续研究寄主植物抗性的有效性提供了动力,寄主植物抗性受到大豆蚜生物型的影响,该生物型克服了含有Rag(对大豆甘氨酸的抗性)基因的大豆植物。在本研究中,通过量化具有Rag基因的大豆基因型和易感品种Williams 82上的蚜虫数量,在无选择分析中评估了在大豆和沙棘(Rhamnus cathatica L.)植物上收集的14个蚜虫无性系以及四个已知的蚜虫生物型(来自我们的库存收集)。使用分离的叶子和整株植物进行无选择测定。通过使用分离的叶子对来自库存收集的生物型的不一致反应证实了全植物分析准确区分生物型的功效。来自伊利诺伊州、印第安纳州和南达科他州的生物型或田间克隆均未克服大豆基因型LD14-8004(Rag1)和PI437696的抗性。来自俄亥俄州Wooster的大豆蚜克隆很容易定殖LD14-8002(Rag2),但没有克服任何其他抗性基因,这表明它与生物型3和4不同,后者也分别克服了LD14-8001和LD14-8006(Rag3)。
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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
12
审稿时长
>12 weeks
期刊介绍: The Journal of the Kansas Entomological Society is a publication of the Kansas (Central States) Entomological Society, publishes research on all aspects of the sciences of entomology, and has world-wide authorship and readership.
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