Effect of pregabalin on fear-based conditioned avoidance learning and spatial learning in a mouse model of scopolamine-induced amnesia

IF 2.8 4区医学 Q2 TOXICOLOGY

Toxicology Mechanisms and Methods Pub Date : 2017-01-08 DOI:10.1080/15376516.2016.1273426

K. Sałat, A. Podkowa, N. Malikowska, J. Trajer

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引用次数: 5

Abstract

Abstract Objectives: Cognitive deficits are one of the frequent symptoms accompanying epilepsy or its treatment. Methods: In this study, the effect on cognition of intraperitoneally administered antiepileptic drug, pregabalin (10 mg/kg), was investigated in scopolamine-induced memory-impaired mice in the passive avoidance task and Morris water maze task. The effect of scopolamine and pregabalin on animals’ locomotor activity was also studied. Results: In the retention phase of the passive avoidance task, pregabalin reversed memory deficits induced by scopolamine (p < 0.05). During the acquisition phase of the Morris water maze pregabalin-treated memory-impaired mice performed the test with longer escape latencies than the vehicle-treated mice (significant at p < 0.05 on Day 5, and at p < 0.001 on Day 6). There were no differences in this parameter between the scopolamine-treated control group and pregabalin-treated memory-impaired mice, which indicated that pregabalin had no influence on spatial learning in this task. During the probe trial a significant difference (p < 0.05) was observed in terms of the mean number of target crossings between vehicle-treated mice and pregabalin-treated memory-impaired mice but there was no difference between the scopolamine-treated control group and mice treated with pregabalin + scopolamine. Pregabalin did not influence locomotor activity increased by scopolamine. Discussion: In passive avoidance task, pregabalin reversed learning deficits induced by scopolamine. In the Morris water maze, pregabalin did not influence spatial learning deficits induced by scopolamine. These results are relevant for epileptic patients treated with pregabalin and those who use it for other therapeutic indications (anxiety, pain).

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普瑞巴林对东莨菪碱致失忆症小鼠基于恐惧的条件回避学习和空间学习的影响

摘要目的：认知缺陷是伴随癫痫或其治疗的常见症状之一。方法：本研究观察腹腔注射抗癫痫药物普瑞巴林（10 mg/kg）在东莨菪碱诱导的记忆受损小鼠中的被动回避任务和Morris水迷宫任务中进行研究。研究了东莨菪碱和普瑞巴林对动物运动活性的影响。结果：在被动回避任务的保留期，普瑞巴林可逆转东莨菪碱引起的记忆缺陷（p < 0.05）。在Morris水迷宫的获取阶段，普瑞巴林处理的记忆受损小鼠比媒介物处理的小鼠具有更长的逃避潜伏期（p < 第5天0.05，p < 0.001）。东莨菪碱治疗的对照组和普瑞巴林治疗的记忆受损小鼠在这一参数上没有差异，这表明普瑞巴林在这项任务中对空间学习没有影响。在探针试验期间 < 0.05），但东莨菪碱处理的对照组和普瑞巴林处理的小鼠之间没有差异 + 东莨菪碱。普瑞巴林不影响东莨菪碱增加的运动活性。讨论：在被动回避任务中，普瑞巴林可逆转东莨菪碱引起的学习缺陷。在Morris水迷宫中，普瑞巴林不影响东莨菪碱引起的空间学习缺陷。这些结果与使用普瑞巴林治疗的癫痫患者以及那些将其用于其他治疗指征（焦虑、疼痛）的患者有关。

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来源期刊

Toxicology Mechanisms and Methods TOXICOLOGY-

自引率

3.10%

发文量

期刊介绍： Toxicology Mechanisms and Methods is a peer-reviewed journal whose aim is twofold. Firstly, the journal contains original research on subjects dealing with the mechanisms by which foreign chemicals cause toxic tissue injury. Chemical substances of interest include industrial compounds, environmental pollutants, hazardous wastes, drugs, pesticides, and chemical warfare agents. The scope of the journal spans from molecular and cellular mechanisms of action to the consideration of mechanistic evidence in establishing regulatory policy. Secondly, the journal addresses aspects of the development, validation, and application of new and existing laboratory methods, techniques, and equipment. A variety of research methods are discussed, including: In vivo studies with standard and alternative species In vitro studies and alternative methodologies Molecular, biochemical, and cellular techniques Pharmacokinetics and pharmacodynamics Mathematical modeling and computer programs Forensic analyses Risk assessment Data collection and analysis.