Effects of biodynamic preparations on concentration of phenolic compounds in the leaves of two white mulberry cultivars

IF 1.4 4区农林科学 Q3 AGRONOMY

Biological Agriculture & Horticulture Pub Date : 2018-10-16 DOI:10.1080/01448765.2018.1535329

E. Jarienė, D. Levickienė, H. Danilčenko, N. Vaitkevičienė, J. Kulaitienė, V. Jakštas, L. Ivanauskas, M. Gajewski

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引用次数: 8

Abstract

ABSTRACT The aim of this study was to evaluate the influence of biodynamic (BD) preparations 500 and 501 on the quality of white mulberry (Morus alba L.) leaves. Leaves of the two mulberry cultivars, Plodovaja 3 and Turchanka, were collected in September 2015 and 2016. Quantitative determinations of phenolic, flavonoid and chlorogenic acid concentrations in mulberry leaves were conducted by UPLC methods. The results showed that the total phenolic compounds concentration (TPCC) and total flavonoid concentration (TFC) in the samples ranged from 4483 to 5005 and from 2944 to 3292 mg ChAE 100 g−1 DM, respectively, with leaves of Turchanka containing the highest concentrations and significant differences between the cultivars for treatments with BD application. The major flavonoids in the mulberry leaves were quercetin-acetylhexoside, isoquercitrin, rutin, kaempferol-acetylhexoside and astragalin, and the concentration of these varied depending on the cultivar (apart from for isoquercitrin and astragalin). The results demonstrated that BD preparations influenced the TPCCs of the two cultivars differently. The application of BD preparation 500 significantly increased the total phenolic and flavonoid concentrations in the leaves of Turchanka by 3.62% and 6.1%, respectively. Using the two preparations together (500 + 501) had significant effects on quercetin-acetylhexoside and kaempferol-acetylhexoside accumulation in the mulberry leaves of both cultivars. Application of preparation 501 significantly reduced the TPCC, TFC and chlorogenic acid concentration in the leaves of Plodovaja 3, compared with the control. The results showed that BD preparations had the greatest positive effect on the accumulation of phenolic compounds in the leaves of the Turchanka cultivar.

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生物动力制剂对两个白桑品种叶片中酚类化合物浓度的影响

摘要本研究旨在评价生物动力制剂500和501对白桑叶质量的影响。两个桑树品种Plodovaja 3和Turchanka的叶子分别于2015年和2016年9月采集。采用UPLC法测定桑叶中酚类、黄酮类和绿原酸的含量。结果表明，样品中总酚类化合物浓度（TPCC）和总类黄酮浓度（TFC）分别为4483至5005和2944至3292 mg ChAE 100 g−1 DM，其中，施用BD处理的Turchanka叶片浓度最高，品种间差异显著。桑叶中的主要黄酮类化合物是槲皮素乙酰己糖苷、异槲皮素、芦丁、山奈酚乙酰己糖苷和黄芪甲素，这些黄酮类化合物的浓度因品种而异（异槲皮素和黄芪甲苷除外）。结果表明，BD制剂对两个品种TPCC的影响不同。BD制剂500的施用显著提高了香蒲叶片中总酚类和黄酮类的含量，分别提高了3.62%和6.1%。两种制剂（500+501）合用对两个品种桑叶中槲皮素乙酰己糖苷和山奈酚乙酰己糖苷的积累均有显著影响。与对照相比，制剂501的施用显著降低了普洛多瓦贾3号叶片中的TPCC、TFC和绿原酸浓度。结果表明，BD制剂对土香卡品种叶片中酚类化合物的积累有最大的积极作用。

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来源期刊

Biological Agriculture & Horticulture 农林科学-农艺学

CiteScore

3.30

自引率

6.70%

发文量

审稿时长

>36 weeks

期刊介绍： Biological Agriculture & Horticulture aims to act as the central focus for a wide range of studies into alternative systems of husbandry, and particularly the biological or organic approach to food production. The Journal publishes work of a sound scientific or economic nature related to any aspect of biological husbandry in agriculture, horticulture and forestry in both temperate and tropical conditions, including energy and water utilization, and environmental impact.