Evaluation of mini-FLOTAC method for diagnosing intestinal parasitic infections

Pub Date : 2019-08-01 DOI:10.21608/puj.2019.13212.1045

Nada A. El-Nadi, A. Ahmed, N. Ahmed, Amany El-Laah

{"title":"Evaluation of mini-FLOTAC method for diagnosing intestinal parasitic infections","authors":"Nada A. El-Nadi, A. Ahmed, N. Ahmed, Amany El-Laah","doi":"10.21608/puj.2019.13212.1045","DOIUrl":null,"url":null,"abstract":"Background: Intestinal parasitic infections (IPIs) are widely distributed throughout the world and have been identified as one of the most significant causes of illnesses and diseases. Accurate diagnosis is essential for proper management and to guide the design, implementation and monitoring of programs for community control of infectious diseases. \nObjective: To evaluate and compare mini-FLOTAC technique with the widely used formol-ethyl-acetate concentration method (FECM) in IPIs detection. \nMaterial and Methods: One hundred fecal samples were randomly collected from different laboratories. All samples were microscopically examined using mini-FLOTAC and FECM. Iodine and modified Zeil-Neelsen stains were used to confirm the presence of protozoa cysts, and oocysts of Cryptosporidium spp. and C. cayetanensis, respectively. \nResults: About two thirds of samples (68%) were positive; 70.6% (48/68) contained only one parasitic infection by at least one method. Generally, the most frequent was Cryptosporidium spp. 35.3% (24/68) followed by E. histolytica/dispar 23.5% (16/68) and G. intestinalis 14.7% (10/68). It was observed that mini-FLOTAC detected the highest number of helminthes infections (100% sensitivity) in contrast to 41.1% for protozoa whereas FECM was the most sensitive approach for protozoa infections (98.2% sensitivity) in contrast to 68.7% for helminthes. \nConclusion: Mini-FLOTAC is a safe rapid device for microscopic examination of stool samples with high sensitivity, affordability, and appropriateness of diagnosis in resource-limited settings where IPIs are widespread","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/puj.2019.13212.1045","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 3

Abstract

Background: Intestinal parasitic infections (IPIs) are widely distributed throughout the world and have been identified as one of the most significant causes of illnesses and diseases. Accurate diagnosis is essential for proper management and to guide the design, implementation and monitoring of programs for community control of infectious diseases. Objective: To evaluate and compare mini-FLOTAC technique with the widely used formol-ethyl-acetate concentration method (FECM) in IPIs detection. Material and Methods: One hundred fecal samples were randomly collected from different laboratories. All samples were microscopically examined using mini-FLOTAC and FECM. Iodine and modified Zeil-Neelsen stains were used to confirm the presence of protozoa cysts, and oocysts of Cryptosporidium spp. and C. cayetanensis, respectively. Results: About two thirds of samples (68%) were positive; 70.6% (48/68) contained only one parasitic infection by at least one method. Generally, the most frequent was Cryptosporidium spp. 35.3% (24/68) followed by E. histolytica/dispar 23.5% (16/68) and G. intestinalis 14.7% (10/68). It was observed that mini-FLOTAC detected the highest number of helminthes infections (100% sensitivity) in contrast to 41.1% for protozoa whereas FECM was the most sensitive approach for protozoa infections (98.2% sensitivity) in contrast to 68.7% for helminthes. Conclusion: Mini-FLOTAC is a safe rapid device for microscopic examination of stool samples with high sensitivity, affordability, and appropriateness of diagnosis in resource-limited settings where IPIs are widespread

查看原文

微型flotac方法诊断肠道寄生虫感染的评价

背景：肠道寄生虫感染（IPIs）在世界各地广泛分布，已被确定为疾病的最重要原因之一。准确的诊断对于正确的管理以及指导社区传染病控制计划的设计、实施和监测至关重要。目的：评价和比较微型FLOTAC技术和广泛使用的甲醇-乙酸乙酯浓度法（FEMM）在IPIs检测中的作用。材料和方法：从不同的实验室随机抽取100份粪便样本。使用迷你FLOTAC和FECM对所有样品进行显微镜检查。碘和改良Zeil-Nelsen染色分别用于确认隐孢子虫和C.cayetanensis的原生动物包囊和卵囊的存在。结果：约三分之二的样本（68%）呈阳性；70.6%（48/68）通过至少一种方法仅包含一种寄生虫感染。一般来说，最常见的是隐孢子虫。35.3%（24/68），其次是溶组织E.dispar 23.5%（16/68）和肠道G.intestinalis 14.7%（10/68）。据观察，迷你FLOTAC检测到的蠕虫感染数量最高（100%的敏感性），而原生动物的感染率为41.1%，而FEMM是原生动物感染最敏感的方法（98.2%的敏感性）。结论：Mini-FLOTAC是一种安全、快速的粪便样本显微镜检查设备，在IPI广泛存在的资源有限的环境中具有高灵敏度、可负担性和适当的诊断

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文