Effect of long-chain non-coding RNA TUG1 on radiosensitivity of human cervical cancer XB1702 cells by adsorption of miR-145

中华放射肿瘤学杂志 Pub Date : 2019-12-15 DOI:10.3760/CMA.J.ISSN.1004-4221.2019.12.013

Xiuling Liu, Senlin Wang, Zhihong Wang, Jing Li, Xinyu Chen, Quanqin He

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引用次数: 0

Abstract

Objective To evaluate the effect of long-chain non-coding RNA TUG1 on the radiosensitivity of cervical cancer cells and explore its underlying mechanism. Methods The expression of TUG1 and miR-145 in cervical cancer cells XB1702 and normal endometrial stromal cells (ESCs) was detected by qRT-PCR. The transfected si-NC, transfected si-TUG1, transfected si-NC combined with irradiation, transfected si-TUG1 combined with irradiation, si-TUG1 and anti-miR-NC co-transfected and, si-TUG1 and anti-miR-145 co-transfected groups were established, which were transfected into XB1702 cells by liposome method. The survival fraction of each group was detected by colony formation assay. The cell apoptosis of each group was detected by flow cytometry. The fluorescence activity of each group was assessed by dual luciferase reporter gene assay. Results Compared with the normal ESCs, the expression of TUG1 was significantly up-regulated, whereas that of miR-145 was significantly down-regulated in the cervical cancer cells XB1702. Silencing TUG1 significantly increased the survival fraction of XB1702 cells, promoted cell apoptosis and enhanced the radiosensitivity of irradiation to XB1702 cells. TUG1 could target and regulate the expression of miR-145. Suppressing miR-145 reversed the silencing effect of TUG1 on inhibiting proliferation, accelerating apoptosis promotion and enhancing sensitization of XB1702 cells. Conclusions Silencing long-chain non-coding RNA TUG1 can enhance the radiosensitivity of cervical cancer cells. The mechanism may be related to targeting miR-145, which will provide a target for radiotherapy of cervical cancer. Key words: TUG1 gene; miR-145 gene; Radiosensitivity; Cervical cancer cell line

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长链非编码RNA TUG1通过吸附miR-145对人宫颈癌XB1702细胞放射敏感性的影响

目的评价长链非编码RNA TUG1对宫颈癌症细胞放射敏感性的影响，并探讨其作用机制。方法采用qRT-PCR方法检测TUG1和miR-145在子宫颈癌症细胞XB1702和正常子宫内膜间质细胞（ESCs）中的表达。分别建立转染si-NC、转染si-TUG1、转染si-NC-联合照射、转染si-TUG1-联合照射、si-TUG1-和抗-miR-NC共转染、si-TUG1-和抗-miR-145共转染组，采用脂质体法转染XB1702细胞。通过菌落形成试验检测各组的存活率。流式细胞仪检测各组细胞凋亡情况。通过双荧光素酶报告基因测定来评估各组的荧光活性。结果癌症XB1702细胞TUG1表达显著上调，而miR-145表达显著下调。沉默TUG1显著提高了XB1702细胞的存活率，促进了细胞凋亡，并增强了对XB1702的辐射敏感性。TUG1可以靶向并调节miR-145的表达。抑制miR-145逆转了TUG1在抑制XB1702细胞增殖、加速细胞凋亡促进和增强增敏方面的沉默作用。结论沉默长链非编码RNA TUG1能增强宫颈癌症细胞的放射敏感性。该机制可能与靶向miR-145有关，miR-145将为宫颈癌症的放射治疗提供靶点。关键词：TUG1基因；miR-145基因；放射敏感性；癌症子宫颈细胞系

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来源期刊

中华放射肿瘤学杂志

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发文量

6375

期刊介绍： The Chinese Journal of Radiation Oncology is a national academic journal sponsored by the Chinese Medical Association. It was founded in 1992 and the title was written by Chen Minzhang, the former Minister of Health. Its predecessor was the Chinese Journal of Radiation Oncology, which was founded in 1987. The journal is an authoritative journal in the field of radiation oncology in my country. It focuses on clinical tumor radiotherapy, tumor radiation physics, tumor radiation biology, and thermal therapy. Its main readers are middle and senior clinical doctors and scientific researchers. It is now a monthly journal with a large 16-page format and 80 pages of text. For many years, it has adhered to the principle of combining theory with practice and combining improvement with popularization. It now has columns such as monographs, head and neck tumors (monographs), chest tumors (monographs), abdominal tumors (monographs), physics, technology, biology (monographs), reviews, and investigations and research.