Associations of single nucleotide polymorphisms in the bovine prolactin gene with phenotypic traits in beef cattle

Q1 Agricultural and Biological Sciences

Agri Gene Pub Date : 2017-09-01 DOI:10.1016/j.aggene.2017.06.002

Laura Meyer, Jeremy Powell, Bryan Kutz, Michael Looper, A. Hayden Brown Jr., Charles Rosenkrans Jr.

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引用次数: 7

Abstract

Fall-calving Angus-based cows (n = 170 unique cows; 400 calving records during three years) were used to determine prolactin (Prl) genotype and haplotype effects on phenotypic traits. Genomic DNA, from buffy coat, was genotyped at three Prl SNP sites (C1286T, A1134T, and G8398A). Traits of interest were: pre-breeding body condition score (BCS) and weight, Julian calving date, calf birth weight, cow weight and BCS at weaning, calf weaning weight, adjusted 205-day weight and cow efficiency. Hair coat scores were determined each year in May, June, and July. Heterozygous cows at SNP C1286T had a lower (P < 0.05) calving rate when compared to homozygous cows. Calf birth weight was affected (P < 0.05) by genotypes at A1134T. Genotype at G8398A did not affect (P > 0.10) phenotypic traits. Six haplotypes were identified: CAG (n = 107), TAA (n = 173), CTG (n = 50), TTA (n = 32), TAG (n = 50), and TTG (n = 37); n represents total number of records for that haplotype during 3-year study. Calving percentage for CAG cows was greater (P < 0.05) than TTA, TAG, and TTG cows (96 vs. 83%; respectively, CAG vs. mean of TTA, TAG, and TTG). Haplotype CAG cows had earlier hair coat shedding. In addition, CAG cows had a larger calving rate (P < 0.05) and greater (P < 0.05) cow efficiency (45 ± 0.9%) than TTG cows. Cows with Prl haplotype CTG or TAG cow efficiency decreased from ≤ 3 to 4–10 and were missing in ≥ 11 years' group, suggesting those cows may have sustainability issues. Our results suggest that mutations associated with the bovine Prl gene may be useful as early selection tools for replacement cattle.

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牛催乳素基因单核苷酸多态性与肉牛表型性状的关系

秋季产犊的盎格鲁奶牛(n = 170头独特的奶牛;利用3年间400例产犊记录，研究了催乳素基因型和单倍型对表型性状的影响。在3个Prl SNP位点(C1286T、A1134T和G8398A)对褐毛被的基因组DNA进行基因分型。感兴趣的性状为:种前体况评分(BCS)和体重、朱利安产犊日期、犊牛初生重、断奶时奶牛体重和BCS、犊牛断奶体重、调整205日体重和奶牛效率。毛被评分在每年的5月、6月和7月进行测定。SNP为C1286T的杂合子奶牛具有较低的P <0.05)的产犊率。犊牛初生体重受影响(P <A1134T基因型差异0.05)。G8398A基因型不影响P >0.10)表型性状。六个单确认:CAG (n = 107), TAA (n = 173), CTG (n = 50), TTA (n = 32),标签(n = 50),和TTG (n = 37);N表示该单倍型在3年研究期间的记录总数。CAG奶牛产犊率较高(P <0.05)， TTA、TAG和TTG奶牛(96比83%;分别为CAG与TTA、TAG、TTG的平均值)。单倍型CAG奶牛毛脱落较早。此外，CAG奶牛的产犊率较高(P <0.05)及更大(P <0.05)奶牛效率(45±0.9%)高于TTG奶牛。Prl单倍型CTG或TAG奶牛效率从≤3下降到4-10，在≥11年组中缺失，表明这些奶牛可能存在可持续性问题。我们的研究结果表明，与牛Prl基因相关的突变可能是替代牛的早期选择工具。

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Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.