The study of inhibitory effect of compound angelica injection on fibroblast proliferation in tendon adhesion

Abstract

Objective To investigate the inhibitory effect and mechanism of compound angelica injection on the proliferation of human fibroblasts. Methods Compound angelica injection with 10, 20, 40 and 80 mg/ml concentrations was administered on human fibroblasts respectively. MTS method was used to detect the proliferation level of human fibroblasts after 48 and 72 hours. The expression level of TGF-β1 was detected by ELISA. Real-time PCR was used to detect the change level of TGF-β1 after compound angelica injection. Results MTS results showed that the proliferation of fibroblasts was inhibited in 20, 40, 80 mg/ml group (P<0.05); real-time PCR results showed that the transcription level of TGF-β1 in fibroblasts decreased in 10, 20, 40 mg/ml group compared with the control group 48 hours after the treatment of compound angelica injection (P<0.05). ELISA results showed that the expression of TGF-β1 was inhibited with the increase of drug concentration 48 hours after the treatment of compound angelica injection (P<0.05). Conclusion Compound angelica injection can inhibit the expression of TGF-β1 in human fibroblasts, suggesting that it can inhibit the proliferation of human fibroblasts, and the inhibition level is concentration-dependent after a certain period of time, which may affect the occurrence of tendon adhesion. Key words: Fibroblasts; Angelica sinensis; Tendon adhesion; TGF-β1