Metabolite profile of ethanol extract of Curcuma domestica Val. variety Turina-1

Abstract

The metabolite profile of Curcuma domestica Val. variety Turina-1, one of the superior varieties of turmeric was studied by analysing ethanol extract of the variety. The samples for this research were obtained from BPPT Bogor-Indonesia and were extracted using ethanol (96 %) and then analyzed using UPLC-QToF-MS/MS System (Waters), mass spectrometry: XEVO-G2QTOF (Waters), in ESI positive resolution mode, using gradient method with mobile phase: water, formic acid and acetonitrile. The study revealed 13 metabolites viz., Demethoxycurcumin-2 (48.23 %), α-Turmerone (19.623 %), Curcumin (18.550 %), Bisdemethoxycurcumin-3 (9.064 %), Curcumin-1, (1.706 %), and other compounds with amount less than 1 % (Kaempferol 3-O-glucosyl-rhamnosyl-galactoside, Demethoxycurcumin, ar-Turmerone, Bisdemethoxycurcumin, a-Terpinolene, L-Tyrosine, L-Alanine and L-serine). Based on this research, the main metabolite in the ethanol extract of Turina-1 having the potential as antioxidants is the curcuminoids.