Synthesis and evaluation of SPION@CMD@Ser-LTVSPWY peptide as a targeted probe for detection of HER2+ cancer cells in MRI

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
Arash Papi, Rasoul Irajirad, Milad Yousefvand, A. Montazerabadi, Z. Mohammadi
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引用次数: 0

Abstract

Objective(s): Successful detection of tumors in the early stages can significantly increase a patient’s healing process and recovery speed. Conventional imaging techniques usually depend on the tissues’ anatomical structure. Epidermal growth factor receptor-2 (HER-2) is a transmembrane protein with an extracellular ligand-binding domain. HER2 plays an essential role in cell proliferation, differentiation, and survival, and its overexpression is associated with various cancers, especially breast and ovarian cancers. Access to its extracellular domain makes HER2 an ideal target for drug preparation and molecular imaging probes. In this study, a targeted magnetic nanoprobe for molecular imaging of HER2 positive cancers was synthesized, and also its potential as a T2-weighted targeted contrast agent was assessed.Materials and Methods: Superparamagnetic SPION nanoparticles were synthesized using the co-precipitation method in the presence of CMD and were labeled with SLTVSPWY peptide. The SPION@CMD@SLTVSPWY nanocomplex was characterized by TEM, DLS, XRD, AAS, FTIR, EDX, and VSM. The r1 and r2 relaxivities were then calculated using a 1.5 Tesla clinical magnetic field. The cytotoxicity of the nanocomplex was evaluated by MTT assay. Finally, the difference between uptake of targeted nanocomplexes and SPION by the human SKOV-3 cell line (HER2 +) was investigated.Results: The SPION@CMD NPs were synthesized with spherical shape and superparamagnetic behavior. Characterization results confirmed the formation of SPION@CMD@SLTVSPWY.  r2 relaxivity and r2/r1 calculations resulted in suitable values of 313 mM-1s-1 and 8.05 for SPION@CMD@SLTVSPWY, respectively. Increased uptake of targeted nanocomplexed (SPION@CMD@SLTVSPWY) compared to non-targeted NPs (SPION@CMD) was very noticeable visually, and its numerical ratio was 3.51 at a concentration of 0.075 mM.  Conclusion: The targeted synthesized nanocomplex in this study has great potential as a T2 weighted probe contrast agent in MR imaging owing to its appropriate high uptake in HER2 + cells.
SPION@CMD@Ser-LTVSPWY肽在MRI中检测HER2+癌细胞靶向探针的合成及评价
目的:早期成功发现肿瘤可以显著加快患者的愈合进程和恢复速度。传统的成像技术通常依赖于组织的解剖结构。表皮生长因子受体-2 (HER-2)是一种具有细胞外配体结合结构域的跨膜蛋白。HER2在细胞增殖、分化和存活中起着至关重要的作用,其过表达与多种癌症,特别是乳腺癌和卵巢癌有关。进入细胞外结构域使HER2成为药物制备和分子成像探针的理想靶标。本研究合成了一种靶向磁性纳米探针,用于HER2阳性癌症的分子成像,并评估了其作为t2加权靶向造影剂的潜力。材料与方法:采用共沉淀法在CMD存在下合成超顺磁性SPION纳米粒子,并用SLTVSPWY肽进行标记。通过TEM、DLS、XRD、AAS、FTIR、EDX和VSM对SPION@CMD@SLTVSPWY纳米配合物进行了表征。然后用1.5特斯拉的临床磁场计算r1和r2的松弛度。采用MTT法评价纳米复合物的细胞毒性。最后,研究了人SKOV-3细胞系(HER2 +)对靶向纳米复合物和SPION摄取的差异。结果:合成的SPION@CMD纳米粒子具有球形和超顺磁性。表征结果证实了SPION@CMD@SLTVSPWY的形成。r2弛度和r2/r1计算得出SPION@CMD@SLTVSPWY的适宜值分别为313 mM-1s-1和8.05。与非靶向纳米复合物(SPION@CMD)相比,靶向纳米复合物(SPION@CMD@SLTVSPWY)的摄取增加非常明显,在浓度为0.075 mM时,其数值比为3.51。结论:本研究合成的靶向纳米复合物在HER2 +细胞中具有适当的高摄取,因此在MR成像中具有很大的潜力作为T2加权探针造影剂。
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
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0
审稿时长
12 weeks
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