Expression analysis of the genes involved in the virulence of Beauveria bassiana

Q1 Agricultural and Biological Sciences

Agri Gene Pub Date : 2019-12-01 DOI:10.1016/j.aggene.2019.100094

Charbel Al Khoury , Georges Nemer , Jacques Guillot , Afif Abdel Nour , Nabil Nemer

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引用次数: 9

Abstract

The entomopathogenic fungus Beauveria bassiana is used as a natural pest killer in many agricultural systems due to its power of overcoming the host immune system. This is partially due to its genome content that expresses key proteins involved in its virulence. In this study, we used Real-Time PCR technique to analyze the relative expression at 48 and 72 h post inoculation of the Hyd1, Hyd2, Bbchit1, Cdep1, Bbbeas, Bbbsls and Vlp4 genes implicated in the pathogenesis process of an indigenous strain (LTB) and a commercial strain (IND) of B. bassiana. This analysis revealed a higher induction of Bbbeas, Bbbsls and Vlp4 genes by the LTB strain when comparing to the IND strain and the calibrator. Furthermore, a more rapid repression of Hyd1 and Hyd2 gene was notable by LTB strain. No significant difference was recorded by the expression of the Cdep1 gene between the test samples and the control. Finally, a significant difference was recorded with the expression of Bbchit1 gene by the IND strain 72 h post-inoculation. In conclusion, these results suggest that B. bassiana strains have differential expression of virulence genes that could reflect adaptation to their geographical environment and could help classifying their entomopathogenic efficacy accordingly.

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球孢白僵菌毒力相关基因表达分析

由于球孢白僵菌具有克服宿主免疫系统的能力，因此在许多农业系统中被用作天然害虫杀手。这部分是由于其基因组内容表达了与其毒性有关的关键蛋白质。本研究采用Real-Time PCR技术，分析了与 球孢酵母本地菌株(LTB)和商业菌株(IND)致病过程相关的Hyd1、Hyd2、Bbchit1、Cdep1、Bbbeas、Bbbsls和Vlp4基因在接种后48和72 h的相对表达量。结果表明，LTB菌株对Bbbeas、Bbbsls和Vlp4基因的诱导率高于IND菌株和校准物。此外，LTB菌株对Hyd1和Hyd2基因的抑制速度更快。Cdep1基因的表达与对照无显著差异。最后，接种IND菌株72 h后，Bbchit1基因的表达有显著差异。综上所述， 球孢白僵菌菌株的毒力基因表达差异反映了其对地理环境的适应性，并有助于对其昆虫致病力进行分类。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.