The negative effects of soil microorganisms on plant growth only extend to the first weeks

Abstract

Soil biotic communities can strongly impact plant performance. So far, most studies on plant-soil-interactions have estimated the effect of the soil microbial community on plant mass after a fixed duration of plant growth. However, these interactions may change over time and several studies have argued that plant-soil interactions are more important for young seedlings than for older plants. In this paper we ask the question: how long-lasting the effect of the soil microbial community on plant growth is. This is important as the growth rate of a plant is not only determined by the growing conditions but also by the size of the plant itself. Therefore, plant with a reduced growth rate early in life, due to negative effects of the soil microbial community, may increase less in biomass for a much longer period even though the relative growth rates do not differ any longer. We examined the plant growth rates at three stages: early growth (0-21 days), mid growth (22 to 42 days) and late growth (43 to 63 days). We performed two growth experiments with Jacobaea vulgaris lasting 49 and 63 days. Plants were grown in sterilized soil or in sterilized soil inoculated with natural dune soil. In a third experiment, we examined the effect of the timing of soil inoculation prior to planting on the (relative-) growth rate of J. vulgaris plants with four different timing treatments. In all experiments, differences in biomass of plants grown in sterilized soil and inoculated soil (live soil) increased throughout the experiment. Interestingly, linear regression models with ln transformed dry weight against time for younger plants and for older plants in sterilized soil and live soil, respectively, showed that the relative growth rate of plants in the sterilized soil was only significantly higher than that of plants in the live soil in the first two to three weeks. After that period there was no longer a negative effect of the live soil on the relative growth rate of plants. In the third experiment, plant biomass decreased with increasing time between inoculation and planting. Overall, our results show that plants of J. vulgaris grew less well in live soil than in sterilized soil. The negative effects of soil inoculation on plant mass appeared to extend over the whole growth period but arise from the negative effects on relative growth rates that occurred in the first weeks after planting when plants have only less than 5% of the mass they obtained after 42 days. Our study highlights the importance of examining relative growth rates rather than final biomass to estimate the effects of soil microbial communities on plants.