Efecto del ácido alfa lipoico sobre la peroxidación no enzimática en mitocondrias de corazón y cerebro de rata

Abstract

Alphalipoc acid (ala) has been characterized as an efficient antioxidant. It has been proposed as a potential therapeutic agent in the treatment or prevention of different pathologies that may be related to an imbalance of the oxido reductive cell state. The objective of this work was to analyze the sensitivity to non-enzymatic peroxidation (nep) (ascorbate-Fe ++ dependent) in heart and brain mitochondria of rats incubated with an ala solution. nep was evaluated by the chemiluminescence method (cl). When the control samples (without the addition of ascorbate-Fe++) were compared with the ascorbate-Fe++ dependent samples, a significant increase in the light emission. Simultaneously, the mitochondria of both organs were incubated with different concentrations of ala (0.05, 0,15 and 0,25 mg/ml), observing a differential protection. Rat brain mitochondria incubated with doses of 0.15 and 0,25 mg/ml of ala were protected from the effects of nep, while in cardiac mitochondria, protection was only observed with the highest dose of ala (0,25 mg/ml). The cl analysis indicated that rat brain mitochondria were protected more efficiently than rat heart mitochondria. In the latter case, it will be necessary to test new doses of ala to demonstrate the effects on these membranes. In conclusion, ala acted as a protective antioxidant of the membranes of both organs against peroxidative damage.