Dopamine-mimetic-coated polyamidoamine-functionalized Fe3O4 nanoparticles for safe and efficient gene delivery

IF 2.5 4区材料科学 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY

Frontiers of Materials Science Pub Date : 2023-03-06 DOI:10.1007/s11706-023-0637-9

Liang Liu, Chaobing Liu, Zhaojun Yang, Yiran Chen, Xin Chen, Jintao Guan

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Abstract

Fe₃O₄ nanoparticles (NPs) are widely used in the construction of drug and gene delivery vectors because of their particular physicochemical properties. Surface modification can not only reduce the cytotoxicity of Fe₃O₄, but also further improve the biocompatibility and delivery efficiency. In this work, firstly, polydopamine (PDA)-coated Fe₃O₄ NPs (named Fe₃O₄@PDA) were prepared by using the self-polymerization characteristics of dopamine in alkaline environment. Then, polyamidoamine (PAMAM) was modified by the Michael addition reaction to prepare water-soluble core—shell magnetic NPs of Fe₃O₄@PDA@PAMAM, and its potential as gene vector was further evaluated. The results revealed that Fe₃O₄@PDA@PAMAM had the ability to condense and protect DNA, and showed lower cytotoxicity, higher cell uptake and transfection efficiency than those of PAMAM. It has the potential to become a magnetic targeted gene vector for further study.

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多巴胺模拟包被聚酰胺胺功能化Fe3O4纳米颗粒安全高效的基因传递

Fe3O4纳米颗粒因其独特的物理化学性质被广泛应用于构建药物和基因传递载体。表面修饰不仅可以降低Fe3O4的细胞毒性，还可以进一步提高Fe3O4的生物相容性和递送效率。本文首先利用多巴胺在碱性环境下的自聚合特性，制备了聚多巴胺(PDA)包被Fe3O4 NPs(命名为Fe3O4@PDA)。然后，通过Michael加成反应对PAMAM进行修饰，制备Fe3O4@PDA@PAMAM的水溶性核壳磁性NPs，并进一步评价其作为基因载体的潜力。结果表明，Fe3O4@PDA@PAMAM具有浓缩和保护DNA的能力，并且比PAMAM具有更低的细胞毒性，更高的细胞摄取和转染效率。它具有成为磁性靶向基因载体的潜力。

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来源期刊

Frontiers of Materials Science MATERIALS SCIENCE, MULTIDISCIPLINARY-

CiteScore

4.20

自引率

3.70%

发文量

515

期刊介绍： Frontiers of Materials Science is a peer-reviewed international journal that publishes high quality reviews/mini-reviews, full-length research papers, and short Communications recording the latest pioneering studies on all aspects of materials science. It aims at providing a forum to promote communication and exchange between scientists in the worldwide materials science community. The subjects are seen from international and interdisciplinary perspectives covering areas including (but not limited to): Biomaterials including biomimetics and biomineralization; Nano materials; Polymers and composites; New metallic materials; Advanced ceramics; Materials modeling and computation; Frontier materials synthesis and characterization; Novel methods for materials manufacturing; Materials performance; Materials applications in energy, information and biotechnology.